AI Article Synopsis
- The study investigates how ribosomes and initiation factors are involved in translating rabbit-reticulocyte hemoglobin mRNA using different ribosomes.
- Successful synthesis of globin chains has been demonstrated in a cell-free system, highlighting the critical role of initiation factors M(1), M(2), and M(3) for this process.
- Both rabbit liver and reticulocyte ribosomes are found to be similarly effective in translating hemoglobin mRNA; however, liver proteins can only partially substitute for some initiation factors.
Article Abstract
The ribosome and initiation factor requirements for translation of rabbit-reticulocyte hemoglobin mRNA on rabbit reticulocyte ribosomes, reticulocyte ribosomal subunits, and liver ribosomes have been studied. Excellent synthesis of globin chains from exogenous mRNA in the fractionated cell-free system has been achieved. There is a near absolute requirement for each of the initiation factors, M(1), M(2), and M(3) (as well as for the supernatant proteins) for the translation of exogenous mRNA. Liver microsomal wash will partially replace reticulocyte factors M(1) and M(2), but will not replace the requirement for reticulocyte factor M(3). Rabbit liver ribosomes and rabbit reticulocyte ribosomes are equally active in their ability to support the translation of exogenous hemoglobin mRNA.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC389517 | PMC |
| http://dx.doi.org/10.1073/pnas.68.11.2752 | DOI Listing |
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