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Protocol for assessing and visualizing cell microaggregate formation in whole blood by imaging flow cytometry.
STAR Protoc
January 2025
Heinz-Nixdorf-Chair of Biomedical Electronics, TranslaTUM, School of Computation, Information and Technology, TUM, Germany; Munich Institute of Biomedical Engineering, TUM, Germany. Electronic address:
Blood cell aggregates are clinically useful biomarkers in a number of medical disorders. This protocol provides accurate and quantitative analysis of cell aggregates using a small volume of whole blood and imaging flow cytometry. We describe steps for sample collection, staining, and measurement.
View Article and Find Full Text PDF3-Dimensional topographic enamel surface changes after different debonding techniques for aligner attachments: a micro-CT study.
Clin Oral Investig
January 2025
Faculty of Dentistry, Department of Orthodontics , Hacettepe University, Sihhiye, Ankara, 06100, Turkey.
Introduction: To evaluate topographic changes of enamel surface in 3-dimensional after different debonding methods of aligner attachments formed with 2 different composite resins.
Methods: Vertical rectangular attachments were created on 88 premolar teeth and divided into two composite resin groups (Group 1:flowable, Group 2:packable) (N = 44). These were then divided into two subgroups (N = 22) using different debonding methods.
Seasonal Pattern of Endo-β-Mannanase Activity During Germination of , Exhibiting Morphophysiological Dormancy.
Plants (Basel)
January 2025
Department of Interdisciplinary Program in Smart Agriculture, Kangwon National University, Chuncheon 24341, Republic of Korea.
Morphophysiological dormancy (MPD) is considered one of the most primitive dormancy classes among seed plants. While extensive studies have examined the occurrence of endo-β-mannanase in seeds with physiological dormancy (PD) or non-dormancy, little is known about the activity of this enzyme in seeds with MPD. This study aimed to investigate the temporal and spatial patterns of endo-β-mannanase activity during dormancy break and germination.
View Article and Find Full Text PDFDifferential Stress Responses to Rice Blast Fungal Infection Associated with the Vegetative Growth Phase in Rice.
Plants (Basel)
January 2025
Department of Genomics and Evolutionary Biology, National Institute of Genetics, Mishima 411-8540, Japan.
During vegetative growth, plants undergo various morphological and physiological changes in the transition from the juvenile phase to the adult phase. In terms of stress resistance, it has been suggested that plants gain or reinforce disease resistance during the process of maturation, which is recognized as adult plant resistance or age-related resistance. While much knowledge has been obtained about changes in disease resistance as growth stages progress, knowledge about changes in plant responses to pathogens with progressing age in plants is limited.
View Article and Find Full Text PDFPlant Adaptation and Soil Shear Strength: Unraveling the Drought Legacy in .
Plants (Basel)
January 2025
Key Laboratory of Mountain Hazards and Earth Surface Processes, Institute of Mountain Hazards and Environment, Chinese Academy of Sciences, Chengdu 610299, China.
Climate change has led to an increasing frequency of droughts, potentially undermining soil stability. In such a changing environment, the shallow reinforcement effect of plant roots often fails to meet expectations. This study aims to explore whether this is associated with the alteration of plant traits as a response to environmental change.
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