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Development of translationally active cell lysates from different filamentous fungi for application in cell-free protein synthesis.
Enzyme Microb Technol
January 2025
Institute of Biotechnology, Brandenburg University of Technology Cottbus-Senftenberg, Universitätsplatz 1, Senftenberg 01968, Germany. Electronic address:
There is an enormous potential for cell-free protein synthesis (CFPS) systems based on filamentous fungi in view of their simple, fast and mostly inexpensive cultivation with high biomass space-time yields and in view of their catalytic capacity. In 12 of the 22 different filamentous fungi examined, in vitro translation of at least one of the two reporter proteins GFP and firefly luciferase was detected. The lysates showing translation of a reporter protein usually were able to synthesize a functional cell-free expressed unspecific peroxygenase (UPO) from the basidiomycete Cyclocybe (Agrocybe) aegerita.
View Article and Find Full Text PDFOptimizing Transformer-Based Network via Advanced Decoder Design for Medical Image Segmentation.
Biomed Phys Eng Express
January 2025
Shandong University, No. 72, Binhai Road, Jimo, Qingdao City, Shandong Province, Qingdao, 266200, CHINA.
U-Net is widely used in medical image segmentation due to its simple and flexible architecture design. To address the challenges of scale and complexity in medical tasks, several variants of U-Net have been proposed. In particular, methods based on Vision Transformer (ViT), represented by Swin UNETR, have gained widespread attention in recent years.
View Article and Find Full Text PDFPatients with anterior cruciate ligament reconstruction frequently present asymmetries in the sagittal plane dynamics when performing single leg jumps but their assessment is inaccessible to health-care professionals as it requires a complex and expensive system. With the development of deep learning methods for human pose detection, kinematics can be quantified based on a video and this study aimed to investigate whether a relatively simple 2D multibody model could predict relevant dynamic biomarkers based on the kinematics using inverse dynamics. Six participants performed ten vertical and forward single leg hops while the kinematics and the ground reaction force "GRF" were captured using an optoelectronic system coupled with a force platform.
View Article and Find Full Text PDFIs Ockham's razor losing its edge? New perspectives on the principle of model parsimony.
Proc Natl Acad Sci U S A
February 2025
Department of Computer Science, University of Manchester, Manchester M13 9PL, United Kingdom.
The preference for simple explanations, known as the parsimony principle, has long guided the development of scientific theories, hypotheses, and models. Yet recent years have seen a number of successes in employing highly complex models for scientific inquiry (e.g.
View Article and Find Full Text PDFFlexible and Durable Conducting Fabric Electrodes for Next-Generation Wearable Supercapacitors.
ACS Appl Mater Interfaces
January 2025
Department of Physics, School of Electrical and Electronics Engineering, SASTRA Deemed to be University, Thanjavur 613 401, Tamil Nadu, India.
This study presents the fabrication of highly conducting Au fabric electrodes using a layer-by-layer (LBL) approach and its application toward energy storage. Through the ligand-exchange mechanism, the alternating layers of tris(2-aminoethyl)amine (TREN) and gold nanoparticles (Au NPs) encapsulated with tetraoctylammonium bromide (TOABr) ligands (Au-TOABr) were deposited onto the fabric to achieve a highly conducting Au fabric (0.12 Ω/□) at room temperature in just two LBL cycles.
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