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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC223825 | PMC |
| http://dx.doi.org/10.1073/pnas.58.6.2235 | DOI Listing |
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A Study of the Aminoacyl-sRNA Synthetases of Phaseolus vulgaris in Relation to Germination.
Plant Physiol
January 1969
Botany Department, University College London, Gower St., London WC1, England.
Optimum conditions for the extraction and assay by ATP-pyrophosphate exchange of the aminoacyl-sRNA synthetases of the various tissues of french bean (Phaseolus vulgaris) seeds and seedlings are described. Extracts of plumules, after passage through Sephadex G-25, were assayed for synthetase activity using an amino acid mixture as substrate, when a 30 to 100-fold stimulation of exchange above the endogenous level was obtained. This marked enhancement of exchange by added amino acids is largely attributed to the use of dilute extracts.
View Article and Find Full Text PDFThe specific activities of 13 aminoacyl-soluble ribonucleic acid (sRNA) synthetases were measured at various time intervals during the germination of Botryodiplodia theobromae conidiospores. The enzyme activities were low or absent in ungerminated spores, and they increased rapidly as germination proceeded. When extracts of the ungerminated spores were prepared with mortar and pestle, very little or no enzyme activity was detected.
View Article and Find Full Text PDFGenetic mapping of the structural gene for a phenylalanyl-sRNA synthetase in Escherichia coli was accomplished by the use of mutants with temperature-sensitive or p-fluorophenylalanine-resistant enzymes. The structural gene is located at minute 33 of the Taylor-Thoman map of the E. coli chromosome, closely linked to a structural gene (aroD) for one of the enzymes involved in the biosynthesis of the aromatic amino acids, and distant from the known locations of other aminoacyl-sRNA synthetase genes.
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