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Heterologous expression of a recombinant ACE inhibitory peptide LYPVK and its potential antihypertensive action mechanism.
Int J Biol Macromol
January 2025
College of Food Science and Engineering, Ocean University of China, Qingdao 266404, China. Electronic address:
Enzymatic hydrolysis approach is commonly employed for preparation of active peptides, while the limited purity and yield of produced peptides hinder further development of action mechanisms. This study presents the biotechnological approach for the efficient production of recombinant angiotensin converting enzyme (ACE) inhibitory peptide LYPVK and investigates its potential antihypertensive action mechanism. DNA encoding sequence of recombinant peptide was designed to form in tandem, which was expressed in Escherichia coli BL21 (DE3).
View Article and Find Full Text PDFDevelopment of an integrated micro-millisystem for continuous laccase extraction.
Bioresour Technol
January 2025
University of Zagreb Faculty of Chemical Engineering and Technology, Marulićev trg 19, HR-10000 Zagreb, Croatia. Electronic address:
Efforts to reduce the impact of chemical processes on the environment are leading to a shift to enzymatic alternatives, with laccases standing out for their versatile substrate oxidation capabilities. This study addresses the improvement of biocatalytic reactions by deep eutectic solvents (DES), in particular DES-based aqueous two-phase systems (ATPS) for the extraction of biomolecules. Continuous laccase extraction from crude samples was achieved using a DES-based ATPS, which was first optimized in a batch extractor and later intensified in a microextractor.
View Article and Find Full Text PDFNano-Zinc Oxide Can Enhance the Tolerance of Apple Rootstock M9-T337 Seedlings to Saline Alkali Stress by Initiating a Variety of Physiological and Biochemical Pathways.
Plants (Basel)
January 2025
College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China.
Soil salinization severely restricts the growth and development of crops globally, especially in the northwest Loess Plateau, where apples constitute a pillar industry. Nanomaterials, leveraging their unique properties, can facilitate the transport of nutrients to crops, thereby enhancing plant growth and development under stress conditions. To investigate the effects of nano zinc oxide (ZnO NP) on the growth and physiological characteristics of apple self-rooted rootstock M9-T337 seedlings under saline alkali stress, one-year-old M9-T337 seedlings were used as experimental materials and ZnO NPs were used as donors for pot experiment.
View Article and Find Full Text PDFEffects of Multiple Treatments of Formic Acid on the Chemical Properties and Structural Features of Bamboo Powder.
Molecules
January 2025
Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Longpan Road 159, Nanjing 210037, China.
Under mild conditions, formic acid effectively separates the components of lignocellulose, removing the majority of the hemicellulose and lignin from the cellulose. However, it has not yet been determined if multiple treatments with fresh formic acid may totally remove hemicellulose and lignin. In this study, fresh formic acid was used to repeatedly pretreat the bamboo powder, and the effect of multiple treatments on the physicochemical structure of the bamboo powder was investigated using changes in fractions, enzymatic hydrolysis, hydrophilicity, cellulose crystallinity, and lignin structure.
View Article and Find Full Text PDFGenome Sequencing Reveals the Potential of sp. Strain UNJFSC003 for Hydrocarbon Bioremediation.
Genes (Basel)
January 2025
Departamento de Agronomía, Universidad Nacional José Faustino Sánchez Carrión (UNJFSC), Lima 15136, Peru.
Bioremediation induced by bacteria offers a promising alternative for the contamination of aromatic hydrocarbons due to their metabolic processes suitable for the removal of these pollutants, as many of them are carcinogenic molecules and dangerous to human health. Our research focused on isolating a bacterium from the rhizosphere of the tara tree with the ability to degrade polycyclic aromatic hydrocarbons, using draft genomic sequencing and computational analysis. sp.
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