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Building Spatiotemporal Understanding of -Host Interactions.
ACS Infect Dis
January 2025
Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111, United States.
Heterogeneity during (Mtb) infection greatly impacts disease outcome and complicates treatment. This heterogeneity encompasses many facets, spanning local differences in the host immune response to Mtb and the environment experienced by the bacterium, to nonuniformity in Mtb replication state. All of these facets are interlinked and each can affect Mtb susceptibility to antibiotic treatment.
View Article and Find Full Text PDFIsoelectric Point of Metal Oxide Films Formed by Anodization.
Langmuir
January 2025
Chemistry and Structure of novel Materials, University of Siegen, Paul-Bonatz Strasse 9-11, 57068 Siegen, Germany.
The surface charge of metal oxides is an important property that significantly contributes to a wide range of phenomena, including adsorption, catalysis, and material science. The surface charge can be predicted by determining the isoelectric point (IEP) of a material and the pH of a solution. Although there have been several studies of the IEP of metal oxide (nano)particles, only a few have reported the IEP of metal oxide films.
View Article and Find Full Text PDFStructural studies of hydrated rare earth nitrates within the stereoatomic model of crystal structures.
Acta Crystallogr B Struct Sci Cryst Eng Mater
February 2025
MIREA - Russian Technological University, 78 Vernadsky Avenue, Moscow, 119454, Russian Federation.
All crystal structures containing nitrate ions, water molecules and one of the rare earth (RE) metal atoms (La-Lu, Y, Sc) were extracted from the Inorganic Crystal Structure Database. The composition of the identified compounds is analyzed in terms of the number of coordinated and uncoordinated water molecules and nitrate ions. Among the resulting compounds, several isotypic and morphotropic series are observed.
View Article and Find Full Text PDFDECODE enables high-throughput mapping of antibody epitopes at single amino acid resolution.
PLoS Biol
January 2025
Laboratory for Synthetic Biology, RIKEN Center for Biosystems Dynamics Research, Osaka, Japan.
Antibodies are extensively used in biomedical research, clinical fields, and disease treatment. However, to enhance the reproducibility and reliability of antibody-based experiments, it is crucial to have a detailed understanding of the antibody's target specificity and epitope. In this study, we developed a high-throughput and precise epitope analysis method, DECODE (Decoding Epitope Composition by Optimized-mRNA-display, Data analysis, and Expression sequencing).
View Article and Find Full Text PDFPreparation of Hydroxyapatite-Aligned Collagen Sheets and Their Evaluation for Fibroblast Adhesion and Collagen Secretion.
ACS Biomater Sci Eng
January 2025
Department of Materials Science and Engineering, School of Materials and Chemical Technology, Institute of Science Tokyo, 2-12-1 Ookayama, Meguro-ku, Tokyo 152-8550, Japan.
The structure of many native tissues consists of aligned collagen (Col) fibrils, some of which are further composited with dispersed hydroxyapatite (HAp) nanocrystals. Accurately mimicking this inherent structure is a promising approach to enhance scaffold biocompatibility in tissue engineering. In this study, biomimetic sheets composed of highly aligned Col fibrils were fabricated using a plastic compression and tension method, followed by the deposition of HAp nanocrystals on the surface via an alternate soaking method.
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