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Yeast cell wall polysaccharides accelerate yet in-feed antibiotic delays intestinal development and maturation via modulating gut microbiome in chickens.
J Anim Sci Biotechnol
January 2025
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, People's Republic of China.
Background: It is important to promote intestinal development and maturation of chicks for feed digestion and utilization, intestinal health, and disease resistance. This study aimed to investigate the effects of dietary yeast cell wall polysaccharides (YCWP) addition on intestinal development and maturation of chickens and its potential action mechanism.
Methods: 180 one-day-old male Arbor Acres broilers were randomly assigned to three groups containing control (basal diets without any antibiotics or anticoccidial drug), bacitracin methylene disalicylate (BMD)-treated group (50 mg/kg) and YCWP-supplemented group (100 mg/kg).
Injectable in situ forming hydrogel based on carboxymethyl chitosan for sustained release of hyaluronic acid: A viscosupplement for biomedical applications.
Carbohydr Polym
March 2025
Orthopaedic clinic, Mehrad hospital, Tehran, Iran.
The reduction in hyaluronic acid concentration and viscosity in the synovial fluid of patients struggling with osteoarthritis increases the abrasion of articular cartilage. The aim of this study was to design a semi-IPN hydrogel based on genipin-crosslinked carboxymethyl chitosan (CMCh) and glycerol to achieve long-term release of hyaluronic acid. The results showed that hydrogel comprising CMCh (3 % wt.
View Article and Find Full Text PDFHydrolysis of chitin and chitosans by the human chitinolytic enzymes: chitotriosidase, acidic mammalian chitinase, and lysozyme.
Int J Biol Macromol
January 2025
Institute for Biology and Biotechnology of Plants, University of Münster, Schlossplatz 8, 48143 Münster, Germany. Electronic address:
Human chitinolytic enzymes trigger growing interest, not only because a wide range of diseases and allergic responses are linked to chitinous components of pathogens, including their interplay with human enzymes, but also due to the increasing use of chitosans in biomedical applications. Here, we present a detailed side-by-side analysis of the only two human chitinases, chitotriosidase and acidic mammalian chitinase, as well as human lysozyme. By analyzing the cleavage of well-characterized chitosan polymers and defined chitin and chitosan oligomers, we report mild processivity and a quantitative subsite preference typical for GH18 chitinases for chitotriosidase and acidic mammalian chitinase.
View Article and Find Full Text PDFApplication of the Chitooligosaccharides and Fluorescence Polarization Technique for the Assay of Active Lysozyme in Hen Egg White.
Biomolecules
December 2024
Laboratory of Glycoconjugate Chemistry, N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospect 47, 119991 Moscow, Russia.
Article Synopsis
- The study explores a fluorescence polarization assay (FPA) using FITC-labeled oligosaccharide tracers to measure active lysozyme in hen egg white, detecting both enzyme-tracer complexes and tracer splitting.
- The assay allows for simultaneous measurement of lysozyme's chitinase and lectin activities, providing a reliable method for detecting lysozyme in complex biological samples.
- FPA offers advantages over traditional turbidimetry by allowing rapid analysis, using standardized reagents, and not requiring bacterial cells, making it useful for evaluating quality in food products.
Interaction of VO-hydrazonates with lysozyme.
J Inorg Biochem
March 2025
Department of Chemical Sciences, University of Naples Federico II, Complesso Universitario di Monte Sant'Angelo, Via Cintia, I-80126 Napoli, Italy. Electronic address:
Vanadium compounds (VCs) exhibit a broad range of pharmacological properties, with their most significant medical applications being in the treatment of cancer and diabetes. The therapeutic effects and mode of action of VCs may be associated with their ability to bind proteins and, consequently, understanding the VC-protein interaction is of paramount importance. Among the promising VCs, the VO complex with the aroylhydrazone furan-2-carboxylic acid ((3-ethoxy-2-hydroxybenzylidene)hydrazide, hereafter denoted as VC1), deserves attention, since it exhibits cytotoxicity against various cancer cell lines, including HeLa.
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