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Development and validation of recombinant A27L based indirect-ELISA for Sheeppox and Goatpox disease in India.
Vet Res Commun
January 2025
ICAR-National Institute of Veterinary Epidemiology and Disease Informatics (NIVEDI), Post Box No. 6450, Yelahanka, Bengaluru, Karnataka, 560119, India.
Sheeppox and Goatpox are highly contagious transboundary viral diseases of sheep and goats caused by Capripoxvirus, respectively. This study describes the development of indirect ELISA and its serodiagnostic potential as an alternative to the gold standard serum neutralization test (SNT). The homologue of vaccinia virus, ORF 117 (A27L) gene of the Romanian Fenner (RF) strain of Sheeppox virus (SPPV) was used for producing the full-length recombinant A27L (rA27L) protein (∼22 kDa) in a prokaryotic expression system.
View Article and Find Full Text PDFGenetic analysis reveals the genetic diversity and zoonotic potential of Streptococcus dysgalactiae isolates from sheep.
Sci Rep
January 2025
Department of Preclinical Sciences, Institute of Veterinary Medicine, Warsaw University of Life Sciences, Ciszewskiego 8 St, 02-786, Warsaw, Poland.
Streptococcus dysgalactiae (S. dysgalactiae ) is a common pathogen of humans and various animals. However, the phylogenetic position of animal S.
View Article and Find Full Text PDFComparison of different freezing rates on post-thaw viability, proliferation, and stemness of sheep spermatogonial stem cells.
Cryobiology
January 2025
Animal Physiology Division, ICAR-National Institute of Animal Nutrition and Physiology, Adugodi, Bangalore, India-560030.
The application of spermatogonial stem cells (SSC) will be more effective and feasible following the successful cryopreservation and transfer of SSCs in livestock. Like other cells, SSCs are also sensitive to cryoinjury; hence composition of the cryomedia and freezing protocols need to be optimized. The present study aims to optimising the best freezing rates by minimising the ice crystallization and dehydration effect in order to maximize the post-thaw SSCs survivability and stemness characteristics.
View Article and Find Full Text PDFSerological Diagnosis of Infection in Cetaceans by Rapid Serum Agglutination Test and Competitive ELISA with and as Antigens.
Pathogens
January 2025
Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise 'G. Caporale', National Reference Center for Brucellosis, 64100 Teramo, Italy.
Rose Bengal antigen and smooth lipopolysaccharide (s-LPS) were produced from a field strain of ("homologous" antigens) and from the reference strain S99 ("heterologous" antigens); they are currently used for the diagnosis of brucellosis in cattle, water buffaloes, sheep, goats, and pigs, as recommended in the Manual of Diagnostic Tests and Vaccines for Terrestrial Animals of the World Organization for Animal Health (WOAH). "Homologous" and "heterologous" antigens were used in a rapid serum agglutination test (Rose Bengal test, RBT) and a competitive ELISA assay (c-ELISA) to test a panel of sera, blood, and other body fluids (cerebrospinal fluid, pericardial fluid, tracheal fluid, and aqueous humor) collected from 71 individuals belonging to five cetacean species (; ; ; ; and ), which were found stranded on the Italian coastline. Six animals were positive for spp.
View Article and Find Full Text PDFApplication of Citizen Science to Sheep as a Model to Sensitize Young Citizens to Biodiversity, Animal Welfare and the Social Utility of Research.
Animals (Basel)
January 2025
School of Biosciences and Veterinary Medicine, University of Camerino, Via Pontoni 5, 62032 Camerino, Italy.
Citizen science activities were performed using sheep as an animal model and involving 252 students aged between 9 and 11 years. The study focused on three pillars: hill/mountain landscape biodiversity, animal welfare and the social utility of research. Two types of tests-"attitude questionnaires" (AQs) and "maximum performance tests" (MPTs)-were administered.
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