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Arginase Activity Inhibition With Thymoquinone Induces a Hybrid Type of Cell-Death in MDA-MB-231 Cell Line.
J Biochem Mol Toxicol
February 2025
Molecular Immuno-Oncology Laboratory, University of Monastir, Monastir, Tunisia.
Arginase plays a crucial role in the urea cycle; it also has immunosuppressive and pro-tumor effects. The present study aimed to assess the effects of arginase inhibition by thymoquinone (2-Isopropyl-5-methyl-1,4-benzoquinone), an active compound of Nigella sativa, on cell death in the MDA-MB-231 triple-negative breast tumor cell line. Cell viability assays, Western blot analysis, and flow cytometry analysis were used to characterize oxidative stress and cell death.
View Article and Find Full Text PDFA single vector system for tunable and homogeneous dual gene expression in Escherichia coli.
Sci Rep
January 2025
Department of Physical Chemistry, Faculty of Chemistry and Chemical Technology, University of Ljubljana, Ljubljana, Slovenia.
Expression of recombinant genes can be controlled using inducible promoters. However, the most commonly used IPTG- and arabinose-inducible promoters result in an 'all-or-nothing' response, leading to fully induced and uninduced bacterial subpopulations. Here, we investigate whether appropriate modifications to these promoter systems can be combined into a single vector system, enabling homogenous expression of two genes of interest that can be precisely tuned using inducer concentration.
View Article and Find Full Text PDFCharacterization of the key aroma compounds in cigar filler tobacco leaves from different production regions.
Front Plant Sci
December 2024
Key Laboratory in Flavor and Fragrance Basic Research, Zhengzhou Tobacco Research Institute, China National Tobacco Corporation, Zhengzhou, China.
Cigar tobacco leaves exhibited distinct regional characteristics, and aroma compounds were the key substances determining the different style features of cigars. However, the differences in aroma characteristics and the mechanisms of key aroma compound synthesis have not been fully elucidated. This study collected filler tobacco leaves (FTLs) from 5 representative domestic and international production regions.
View Article and Find Full Text PDFQuorum sensing signals of the grapevine crown gall bacterium, sp. Rr2-17: use of inducible expression and polymeric resin to sequester acyl-homoserine lactones.
PeerJ
December 2024
The Thomas H. Gosnell School of Life Sciences, Biotechnology and Molecular Bioscience Program, College of Science, Rochester Institute of Technology, Rochester, New York, United States.
Background: A grapevine crown gall tumor strain, sp. strain Rr2-17 was previously reported to accumulate copious amounts of diverse quorum sensing signals during growth. Genome sequencing identified a single luxI homolog in strain Rr2-17, suggesting that it may encode for a AHL synthase with broad substrate range, pending functional validation.
View Article and Find Full Text PDFSynthesis and Application of Photocaged Isopropyl β-D-1-Thiogalactopyranoside for Light-Mediated Control of Bacterial Gene Expression.
Methods Mol Biol
December 2024
Institute of Molecular Enzyme Technology, Heinrich Heine University Düsseldorf, Forschungszentrum Jülich GmbH, Jülich, Germany.
Photocaged compounds are chemical conjugates that are designed to release an active molecule upon exposure to light of a specific wavelength. In recent years, photocaged inducer molecules such as caged isopropyl β-D-1-thiogalactopyranoside (cIPTG) have been increasingly used as a powerful tool for light-driven gene expression in bacteria, allowing researchers to precisely and noninvasively tune the expression of specific target genes. In this chapter, we present a guideline for the synthesis of 6-nitropiperonyl photocaged IPTG (NP-cIPTG) as well as its in vivo application as an optochemical on-switch of gene transcription in Escherichia coli and other bacteria.
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