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Induction of CYP2C genes in human hepatocytes in primary culture.
Drug Metab Dispos
March 2001
INSERM U128, IFR24, Campus Centre National de la Recherche Scientifique, 1919 Route de Mende, 34293 Montpellier, France.
The expression and inducibility of four CYP2C genes, including CYP2C8, -2C9, -2C18, and -2C19, was investigated in primary cultures of human hepatocytes. By the use of RNase protection assay and specific antibodies, each CYP2C mRNA and protein were quantified unequivocally. The four CYP2C mRNAs were expressed in human livers and cultured primary hepatocytes, but only the CYP2C18 protein was not detected.
View Article and Find Full Text PDF[An analysis of the mechanism of the resistance to barbiturate action by using exogenous RNA].
Biull Eksp Biol Med
September 1993
For the first time the experimental method of exogenous RNA has been used to evaluate the role of protein synthesis in different organs for the development of resistance to the soporific effect of barbiturates. Liver cytosolic RNA of phenobarbital-treated donors was found to reproduce completely the effect of phenobarbital-induced resistance to barbiturates in recipient rats: the reduction of hexenal-induced sleep and the increase of cytochrome P450 content in hepatic microsomes. Brain and renal RNAs had no influence on recipients.
View Article and Find Full Text PDFLiver microsomes, isolated from rats which had been treated with phenobarbital in vivo, were found to exhibit increased activities of oxidative demethylation and TPNH-cytochrome c reductase and an increased amount of CO-binding pigment. Simultaneous administration of actinomycin D or puromycin abolished the phenobarbital-induced enzyme synthesis. Increased rate of P(i) (32) incorporation into microsomal phospholipid was the first sign of phenobarbital stimulation and appeared 3 hours after a single injection of this drug.
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