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Optimizing Re-staining Techniques for the Restoration of Faded Hematoxylin and Eosin-stained Histopathology Slides: A Comparative Study.
J Histochem Cytochem
December 2024
Department of Pathology, Phramongkutklao College of Medicine, Bangkok, Thailand.
Hematoxylin and eosin (H&E)-stained slides inevitably deteriorate over time, frequently becoming unreadable. Reutilizing these slides can reduce the need for additional serial sections, particularly when the target region is no longer available in the tissue block. This study aims to develop efficient protocols for recycling faded H&E-stained slides, providing benefits for future research on stored samples.
View Article and Find Full Text PDFImproving the prediction of protein stability changes upon mutations by geometric learning and a pre-training strategy.
Nat Comput Sci
November 2024
MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing, China.
Accurate prediction of protein mutation effects is of great importance in protein engineering and design. Here we propose GeoStab-suite, a suite of three geometric learning-based models-GeoFitness, GeoDDG and GeoDTm-for the prediction of fitness score, ΔΔG and ΔT of a protein upon mutations, respectively. GeoFitness engages a specialized loss function to allow supervised training of a unified model using the large amount of multi-labeled fitness data in the deep mutational scanning database.
View Article and Find Full Text PDFChromium immobilization from wastewater via iron-modified hydrochar: Different iron fabricants and practicality assessment.
Ecotoxicol Environ Saf
April 2024
School of Energy and Environment Science, Yunnan Normal University, Kunming 650500, China; Agency on Rural Energy Management of Yunnan Province, Kunming 650500, China. Electronic address:
The recycling of industrial solid by-products such as red mud (RM) has become an urgent priority, due to their large quantities and lack of reutilization methods can lead to resource wastage. In this work, RM was employed to fabricate green hydrochar (HC) to prepare zero-valent iron (ZVI) modified carbonous materials, and conventional iron salts (IS, FeCl) was applied as comparison, fabricated HC labeled as RM/HC and IS/HC, respectively. The physicochemical properties of these HC were comprehensively characterized.
View Article and Find Full Text PDFLabel-Free, Reusable, Equipment-Free, and Visual Detection of Hydrogen Sulfide Using a Colorimetric and Fluorescent Dual-Mode Sensing Platform.
Anal Chem
April 2023
Department of Pharmacology, North Sichuan Medical College, Nanchong, Sichuan 637100, P. R. China.
In this work, we have found for the first time that the fluorescence of rhodamine B (RhB) would be dramatically reduced after it bound to hemin/G-quadruplex and reacted with •OH. Based on this finding, we have designed a colorimetric and fluorescent dual-mode sensing platform for visual detection of hydrogen sulfide (HS). The constructed sensor is based on the formation of dsDNA and the G-quadruplex structure by the cytosine-Ag-cytosine mismatch, causing HO-mediated catalysis to oxidize ABTS or RhB to induce a colorimetric or fluorescent change.
View Article and Find Full Text PDFSpns1 is a lysophospholipid transporter mediating lysosomal phospholipid salvage.
Proc Natl Acad Sci U S A
October 2022
Signature Research Program in Cardiovascular and Metabolic Disorders, Duke-National University of Singapore (NUS) Medical School, 169857, Singapore.
The lysosome is central to the degradation of proteins, carbohydrates, and lipids and their salvage back to the cytosol for reutilization. Lysosomal transporters for amino acids, sugars, and cholesterol have been identified, and the metabolic fates of these molecules in the cytoplasm have been elucidated. Remarkably, it is not known whether lysosomal salvage exists for glycerophospholipids, the major constituents of cellular membranes.
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