AI Article Synopsis
- - Researchers studied the synthesis of adenovirus type 2 DNA in HeLa cells, focusing on the intermediates that form during the process.
- - They found smaller DNA fragments after labeling with radioactive thymidine, but the shorter Okazaki fragments did not dominate the results at any of the measured times.
- - The DNA intermediates were confirmed to be of viral origin, produced during the G(2) phase of the cell cycle, when the host DNA is not actively being synthesized.
Article Abstract
Intermediates in the synthesis of adenovirus type 2 deoxyribonucleic acid (DNA) were studied in HeLa cells. Pieces of DNA smaller than the viral genome were demonstrated after labeling with (3)H-thymidine for 10 to 240 sec. Intermediates as small as the Okazaki fragments (8 to 10S) do not predominate at any of the above times. No detectable addition of nucleotides to parental genome could be shown, nor was there any breakdown of recently synthesized viral DNA. The DNA intermediates were of viral origin for they hybridized to viral DNA and were made at a stage of the cell cycle (G(2)) when host DNA is not synthesized.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC376246 | PMC |
| http://dx.doi.org/10.1128/JVI.8.5.675-683.1971 | DOI Listing |
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