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Cockroaches as Reservoirs, Vectors, and Potential Sentinels of Multidrug-Resistant Bacteria in Ugandan Communities: A Retrospective Analysis.
Glob Health Epidemiol Genom
January 2025
Center for Comparative Epidemiology, College of Veterinary Medicine, Michigan State University, 736 Wilson Road, Room A109, East Lansing, Michigan 48824, USA.
Cockroaches could play a role in the transmission dynamics of antimicrobial-resistant bacteria (ARB) at variable interfaces in Ugandan communities, acting as both reservoirs and vectors. This study investigated the burden and diversity of ARB carried by cockroaches in human settlements in Uganda, so as to understand their role in the spread of these pathogens and their potential as sentinels in antimicrobial resistance (AMR) surveillance programs. A retrospective analysis was conducted on two unpublished studies by Makerere University students.
View Article and Find Full Text PDFAssessing hard and loose "endpoints": comparison of patient and expert Bristol Stool Scale scoring of 2280 fecal samples.
F1000Res
January 2025
Unger-Vetlesen Institute, Lovisenberg Diaconal Hospital, Oslo, Oslo, Norway.
Background: Stool consistency is an important outcome measure to evaluate in the investigation of several gastrointestinal diseases. The Bristol Stool Scale (BSS) is one of the most commonly used tools for evaluation of stool consistency. BSS ranges from 1-7 and each score is assigned to a given consistency of the feces.
View Article and Find Full Text PDFAddressing the overuse of hospital emergency departments in the Portuguese NHS: a new paradigm.
Front Public Health
January 2025
Portuguese National Health Service Executive Board, Porto, Portugal.
The escalating trend of inappropriate visits to Emergency Departments (ED) has led to significant concerns, including resource misallocation, compromised patient care, and an increased burden on healthcare workers. Portugal faces a notable challenge, reporting one of the highest ED visit rates, with an annual average of approximately 6 million ED visits from 2013 to 2023. In response, the "Call First, Save Lives" pilot project was launched by the Portuguese NHS Executive Board, in 2023, at the Local Health Unit (LHU) of Póvoa de Varzim/Vila do Conde.
View Article and Find Full Text PDFIn this work, we investigated individual bacteria belonging to strains of the Beijing family with different drug sensitivity (sensitive, multi and extensive drug-resistant) by surface-enhanced Raman spectroscopy (SERS) in the fingerprint region. The latter is focused on the spectral bands, which correspond to a set of glutathione bands and DNA methylation patterns revealed due to 5-methylcytosine spectral biomarkers. It is shown that these spectral features can be correlated with drug sensitivity and DNA methylation.
View Article and Find Full Text PDFSynthesis and Characterization of MIP Ghost Approach for Selective Extraction of Empagliflozin and Quantification by Liquid Chromatography.
J Chromatogr Sci
January 2025
Department of Chemistry & Biochemistry, Ohio University, Athens, OH, USA.
The valid method was developed for analyzing empagliflozin in serum/plasma/urine using a molecularly imprinted ghost polymer-solid-phase extraction approach (MISPE) with liquid chromatographic methodology. Methacrylic acid (MAA) was used as the monomer, 2,2 azobis isobutyronitrile as the initiator and ethylene glycol dimethacrylate as the cross-linker in the free radical polymerization procedure. Empagliflozin was loaded onto the polymer and eluted with 1 mL of a 9:1 MeOH:acetic acid solution.
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