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Epididymal bull sperm selection by Percoll® density-gradient centrifugation prior to conventional or ultra-rapid freezing enhances post-thaw sperm quality.
Cryobiology
January 2025
Laboratorio de Biotecnología de la Reproducción Animal, Facultad de Ciencias Agropecuarias, Universidad de Cuenca, EC010205, Cuenca, Ecuador. Electronic address:
This study evaluated the effectiveness of Percoll® density gradient centrifugation (Percoll-DGC) for selecting bull epididymal sperm prior to conventional slow (CS) or ultra-rapid (UR) freezing and its effects on sperm quality. Fifteen pooled samples from 30 epididymides (2 different samples/pool) of 15 bulls were split into two aliquots assigned to either CS or UR freezing. Samples were either selected using Percoll-DGC (40/80 %) or left non-selected (control), resulting in four pre-freezing treatments: Percoll-CS, Control-CS, Percoll-UR, and Control-UR.
View Article and Find Full Text PDFThe effect of myo-inositol antioxidant activity on human sperm parameters and DNA damage in ultra-rapid and conventional freezing methods.
Cryobiology
December 2024
Reproductive Sciences and Technology Research Center, Department of Anatomical Sciences, School of Medicine, Iran University of Medical Sciences, Tehran, Iran; Shahid Akbarabadi Clinical Research Development Unit (ShACRDU), Iran University of Medical Sciences, Tehran, Iran. Electronic address:
Article Synopsis
- Male fertility preservation faces issues like cell damage from sperm freezing and challenges in maintaining sperm quality post-thawing, especially with traditional freezing techniques.
- This study compared different freezing methods, including conventional freezing and ultra-rapid freezing, with and without an antioxidant called myo-inositol, to see how well sperm survives the freezing process.
- Results showed that ultra-rapid freezing with myo-inositol resulted in the best sperm morphology and the least DNA damage, indicating that modified freezing methods can enhance sperm preservation.
Model-Based Optimization of Solid-Supported Micro-Hotplates for Microfluidic Cryofixation.
Micromachines (Basel)
August 2024
Integrated Micro-Nano-Systems Laboratory, Technische Universität Darmstadt, 64283 Darmstadt, Germany.
Cryofixation by ultra-rapid freezing is widely regarded as the gold standard for preserving cell structure without artefacts for electron microscopy. However, conventional cryofixation technologies are not compatible with live imaging, making it difficult to capture dynamic cellular processes at a precise time. To overcome this limitation, we recently introduced a new technology, called microfluidic cryofixation.
View Article and Find Full Text PDFL-carnitine supplementation in conventional slow and ultra-rapid freezing media improves motility, membrane integrity, and fertilizing ability of dog epididymal sperm.
Anim Reprod Sci
November 2024
Laboratorio de Biotecnología de la Reproducción Animal, Facultad de Ciencias Agropecuarias, Universidad de Cuenca, Cuenca EC010205, Ecuador; Centro Latinoamericano de formación en especies mayores y menores - CLAFEM, Cuenca, Ecuador. Electronic address:
This study aimed to assess the impact of L-carnitine (LC) supplementation in conventional-slow (CS) and ultra-rapid (UR) freezing media on post-thaw quality and fertilizing ability of dog epididymal spermatozoa. Sperm samples were collected from 60 epididymides obtained from 30 adult orchiectomized dogs via retrograde flushing. Twenty pooled sperm samples were then created (3 epididymal samples/pool).
View Article and Find Full Text PDFVitrification of Ovarian Cortex Tissue to Achieve a Glassy State of Aggregation.
J Vis Exp
August 2024
Department of Gynecological Endocrinology and Reproductive Medicine, University Hospital of Bonn;
Ovarian tissue cryopreservation (OTC) is an important option for fertility preservation. For patients whose gonadotoxic treatments cannot be postponed or for pre-pubertal girls, it is often the only option for fertility protection. Cryopreservation can be performed either by vitrification or by slow freezing.
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