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Bta-miR-93 regulates the maternal-fetal tolerance in dairy cows via promoting PD-L1-mediated M2 macrophage polarization†.
Biol Reprod
March 2025
Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
Bovine embryo resorption is one of the key factors restricting the reproductive efficiency in dairy cattle, which mainly occurs in the early stage of maternal recognition of pregnancy (MRP), causing substantial economic losses to the dairy industry. Macrophages, the most numerous endometrial immune cells in cows during early pregnancy, are critical in developing maternal-fetal immune tolerance. However, the mechanism of their action on the maternal-fetal interface of dairy cows remains unknown.
View Article and Find Full Text PDFRodent surgeries: who is performing them and how?
Lab Anim
March 2025
Zurich Integrative Rodent Physiology (ZIRP), Institute of Physiology, University of Zurich, Zurich, Switzerland.
Surgery is an integral part of many experimental studies. Good surgical practice is a prerequisite for surgical success, optimal animal welfare, and it not only improves post-operative recovery but also the overall outcome and validity of a study. Rodents, especially mice, are the most commonly used laboratory animals and the legal requirements to perform experimental surgery are identical for all species.
View Article and Find Full Text PDFBroiler litter moisture and trace metals contribute to the persistence of strains that harbor large plasmids carrying siderophores.
Appl Environ Microbiol
March 2025
U.S. National Poultry Research Center, USDA-ARS, Athens, Georgia, USA.
Broiler litter sampling has proven to be an effective method for determining the status of a broiler chicken flock and understanding the ecology of prior to harvest. In this study, we investigated the ecology of within the litter ( = 224) from two commercial broiler houses in the United States. We employed culture enrichment methods and quantitative polymerase chain reaction to determine the prevalence and load of and utilized antimicrobial susceptibility testing and whole-genome sequencing (WGS) to characterize select isolates.
View Article and Find Full Text PDFNongenomic Progesterone Receptors Mediated Bull Sperm Capacitation and Acrosome Reaction Are cAMP-PKA-Calcium Channel Dependent.
Reprod Domest Anim
March 2025
College of Veterinary Science and Animal Husbandry, UP Pandit Deen Dayal Upadhaya Pashuchikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan, Mathura, India.
Progesterone (P4) secreted from the cumulus cells mediates nongenomic signalling in sperm cells and induces capacitation and acrosome reaction in the oviduct. The induction of these events in bull spermatozoa is not fully understood. Recently, we reported the presence of membrane nongenomic P4 receptors in bull spermatozoa and the involvement of P4-nongenomic signalling through the involvement of complex signalling pathways with cannabinoid receptors in the regulation of bull sperm capacitation and acrosome reaction.
View Article and Find Full Text PDFIn vitro and in vivo antibacterial activity, resistance analysis and molecular docking study of pleuromutilin derivatives against Streptococcus suis.
J Antimicrob Chemother
March 2025
Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, P.R.China.
Objectives: To evaluate the in vitro and in vivo antimicrobial activity of pleuromutilin derivatives modified with C14 side-chain against Streptococcus suis.
Methods: To determine the minimum inhibitory concentrations (MICs) of 268 pleuromutilin derivatives with C14 side-chain modifications against S. suis ATCC 43 765 using the broth dilution method.
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