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Determination of the optimal method for measuring malondialdehyde in human saliva.
MethodsX
June 2025
Centre des Sciences du Goût et de l'Alimentation, CNRS, INRAE, Institut Agro, Université de Bourgogne, F-21000 Dijon, France.
While few methodological studies have been published on the salivary measurement of malondialdehyde (MDA), none have precisely detailed the analytical method. This work presents the development of an analytical method for MDA measurement in microvolumes of saliva samples from healthy individuals, using thiobarbituric acid derivatization and fluorescence reading of the formed compound. This method was progressively designed to meet specific constraints such as the limited sample volume available, cost-effectiveness of each assay, time required for analysis and costs.
View Article and Find Full Text PDFOptical Methods for Determining the Phagocytic Activity Profile of CD206-Positive Macrophages Extracted from Bronchoalveolar Lavage by Specific Mannosylated Polymeric Ligands.
Polymers (Basel)
December 2024
Faculty of Chemistry, Lomonosov Moscow State University, Leninskie Gory, 1/3, 119991 Moscow, Russia.
Macrophage (Mph) polarization and functional activity play an important role in the development of inflammatory lung conditions. The previously widely used bimodal classification of Mph into M1 and M2 does not adequately reflect the full range of changes in polarization and functional diversity observed in Mph in response to various stimuli and disease states. Here, we have developed a model for the direct assessment of Mph from bronchial alveolar lavage fluid (BALF) functional alterations, in terms of phagocytosis activity, depending on external stimuli, such as exposure to a range of bacteria (, and ).
View Article and Find Full Text PDFDamsin and neoambrosin: Two sesquiterpene lactones with affinity and different activity for PPAR and TRPA1 receptors.
Bioorg Chem
January 2025
NEUROFARBA Department, Pharmaceutical and Nutraceutical Section and Laboratory of Molecular Modeling Cheminformatics & QSAR, University of Florence, Via U. Schiff 6, Sesto Fiorentino, Florence 50019, Italy. Electronic address:
Ambrosia maritima L. (family Asteraceae) is an annual herb widely distributed throughout the Mediterranean region and Africa. The herb is employed in folk medicine for the treatment of many ailments.
View Article and Find Full Text PDFSmall-Molecule Probe for Imaging Oxidative Stress-Induced Carbonylation in Live Cells.
Bio Protoc
November 2024
Gottfried Schatz Research Center for Cell Signaling, Metabolism and Aging, Department of Biophysics, Medical University of Graz, Neue Stiftingtalstrasse, Graz, Austria.
Article Synopsis
- - Protein carbonylation is a key irreversible modification that indicates oxidative stress but lacks distinct spectroscopic properties, making it challenging to detect without specific chemical probes.
- - The protocol details the synthesis of a new fluorescent probe, 2Hzin5NP, which reacts rapidly with carbonyls to create a detectable product, and outlines the methods to evaluate its effects on cell health and carbonylation.
- - The probe can differentiate carbonylation profiles in response to oxidative stress in healthy and cancerous cells, providing insight into varying oxidative stress levels between these conditions.
The Kinetics of Carbon-Carbon Bond Formation in Metazoan Fatty Acid Synthase and Its Impact on Product Fidelity.
Angew Chem Int Ed Engl
January 2025
Institute of Organic Chemistry and Chemical Biology, Buchmann Institute of Molecular Life Sciences, Goethe University Frankfurt, Max-von-Laue-Str. 15, 60438, Frankfurt am Main, Germany.
Fatty acid synthase (FAS) multienzymes are responsible for de novo fatty acid biosynthesis and crucial in primary metabolism. Despite extensive research, the molecular details of the FAS catalytic mechanisms are still poorly understood. For example, the β-ketoacyl synthase (KS) catalyzes the fatty acid elongating carbon-carbon-bond formation, which is the key catalytic step in biosynthesis, but factors that determine the speed and accuracy of his reaction are still unclear.
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