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A novel microassay for measuring blood alcohol concentration using a disposable biosensor strip.
Forensic Sci Int
April 2011
Key Laboratory of Laboratory Medical Diagnostics of Education, Department of Laboratory Medicine, Chongqing Medical University, No. 1 Yi Xue Yuan Road, Chongqing 400016, China.
A novel disposable amperometric biosensor strip for determination of blood alcohol concentration (BAC) with small volume of sample has been constructed using screen-printed electrodes (SPE), nanocomposite film and alcohol dehydrogenase (ADH). Firstly, the GNPs-MWCNT-Nafion nanocomposite film modified on a working electrode was made of Nafion-117, multi-wall carbon nanotubes (MWCNT) and gold nanoparticles (GNPs). After that Meldola's blue (MB) acted as an electron transfer mediator and the mixed solution of ADH, nicotinamide adenine dinucleotide (NAD(+)) were modified in order on the nanocomposite film.
View Article and Find Full Text PDFA combined analysis of regional energy metabolism and immunohistochemical ischemic damage in the gerbil brain.
J Neurochem
March 1999
Department of Neurology, Mayo Clinic and Mayo Foundation, Rochester, Minnesota, USA.
By combining immunohistochemical technique with microassay methods, we analyzed regional energy metabolism in vulnerable and tolerant areas of gerbil brains during evolution of neuronal damage after bilateral common carotid artery occlusion for 10 min with subsequent reperfusion. Four animals were used for each reperfusion period. Based on the information from the immunohistochemical examination, we dissected out vulnerable and tolerant subregions of the hippocampus, cerebral cortex, and thalamus from freeze-dried 20-microm-thick sections, and measured the levels of creatine phosphate (P-Cr), adenine nucleotides, guanine nucleotides, and purine bodies by HPLC, and the levels of glucose, glycogen, and lactate by an enzyme-immobilized column method.
View Article and Find Full Text PDFDifferences in the chemical reactivity of individual molecules of an enzyme.
Nature
February 1995
Ames Laboratory-USDOE, Iowa State University, Ames 50011.
Much attention has been focused recently on the detection and physical characterization of individual molecules. Using such methods to study the chemical properties, such as reactivity, of single molecules offers the potential to investigate how these might vary from molecule to molecule, and for individual molecules as a function of time. The complex structures of biomolecules such as enzymes make them particularly attractive targets for studying how subtle changes or differences at the molecular level might influence chemical reactivity.
View Article and Find Full Text PDFHPRT activity in embryos of a South American opossum Monodelphis domestica.
Reprod Fertil Dev
April 1995
School of Biological Sciences, Macquarie University, Sydney, NSW, Australia.
Marsupial females show preferential paternal X-inactivation. However, the time at which X-inactivation occurs in early development has not yet been determined. A double microassay which measures the activities of X-linked hypoxanthine phosphoribosyl transferase (HPRT) and the autosomally-coded adenine phosphoribosyl transferase (APRT) from the same sample was performed on a collection of embryos from a South American opossum Monodelphis domestica.
View Article and Find Full Text PDFHypoxanthine phosphoribosyltransferase activity in bovine embryos during the early embryonic development.
Theriogenology
August 1993
Ishikawa Prefectural Livestock Experiment Station, Oshimizu-cho, Hakui, Ishikawa, 929-13 Japan.
The activity of hypoxanthine phosphoribosyltransferase (HPRT) was determined in the bovine embryo during early embryonic development. Microassay, using [(3)H] hypoxanthine, was improved to measure enzyme activity in the embryonic extract. This activity depended on the reaction time and the concentration of phosphorybosyl pyrophosphate (PRPP) in a reaction.
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