In solutions of creatine kinase, actin and lactic dehydrogenase oscillations of SH-groups are revealed by three independent methods using three different reagents AgNO3, PCMB, DTNB. The variations of the SH-group titre remain after denaturation of the individual protein solution portions. In the solutions of predenaturated proteins no titre oscillations are observed. Possible cause of the observed oscillations of SH-groups titre may be reversible SH--SS transformation.
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Modulation of Na/K- ATPase activity by triterpene 3β, 6β, 16β-trihidroxilup-20 (29)-ene (TTHL) limits the long-term secondary degeneration after traumatic brain injury in mice.
Eur J Pharmacol
July 2019
Centro de Ciências da Saúde, Programa de Pós-Graduação em Farmacologia - Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, Brazil; Centro de Educação Física e Desportos, Departamento de Métodos e Técnicas Desportivas, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, Brazil. Electronic address:
Traumatic brain injury (TBI) is a public health problem characterized by a combination of immediate mechanical dysfunction of the brain tissue, and secondary damage. Based on the hypothesis that selected targets, such as Na K-ATPase are involved in the secondary damage after TBI and modulation of this enzyme activity by triterpene 3β, 6β, 16β-trihidroxilup-20 (29)-ene (TTHL) supports the ethnomedical applications of this plant, we decided to investigate whether previous TTHL treatment interrupts the progression of pathophysiology induced by TBI. Statistical analyses revealed that percussion fluid injury (FPI) increased Na,K-ATPase activity in all isoform (α and α) 15 min after neuronal injury.
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Cytobios
November 1987
Department of Biophysics, University of Lódź, Poland.
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View Article and Find Full Text PDFIn solutions of creatine kinase, actin and lactic dehydrogenase oscillations of SH-groups are revealed by three independent methods using three different reagents AgNO3, PCMB, DTNB. The variations of the SH-group titre remain after denaturation of the individual protein solution portions. In the solutions of predenaturated proteins no titre oscillations are observed.
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J Gen Physiol
March 1943
McArdle Memorial Laboratory, University of Wisconsin Medical School, Madison.
1. The mechanism of succinic dehydrogenase action was studied by means of inhibitors. 2.
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