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Highly sensitive two-dimensional profiling of N-linked glycans by hydrophilic interaction liquid chromatography and dual stacking capillary gel electrophoresis.
Anal Chim Acta
September 2024
Department of Chemistry, Graduate School of Science, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, 819-0395, Japan; RIKEN Center for Biosystems Dynamics Research, 6-2-4 Furuedai, Suita, Osaka, 565-0874, Japan. Electronic address:
Background: N-Glycosylation is one of the most important post-translational modifications in proteins. As the N-glycan profiles in biological samples are diverse and change according to the pathological condition, various profiling methods have been developed, such as liquid chromatography (LC), capillary electrophoresis (CE), and mass spectrometry. However, conventional analytical methods have limitations in sensitivity and/or resolution, hindering the discovery of minor but specific N-glycans that are important both in the basic glycobiology research and in the medical application as biomarkers.
View Article and Find Full Text PDFPicomolar detection limits for glyphosate by two-dimensional column-coupled isotachophoresis/capillary zone electrophoresis-mass spectrometry.
J Sep Sci
October 2022
Institute of Physical and Theoretical Chemistry, Department of Chemistry, Eberhard Karls Universität Tübingen, Tübingen, Germany.
Capillary electrophoresis-mass spectrometry often lacks sufficient limits of detection for trace substances in the environment due to its low loadability. To overcome this problem, we conducted a feasibility study for column-coupling isotachophoresis to capillary electrophoresis-mass spectrometry. The first dimension isotachophoresis preconcentrated the analytes.
View Article and Find Full Text PDFComparison of 1D a 2D ITP-MS performance parameters and application possibilities: Ultratrace determination of B vitamins in human urine.
Electrophoresis
May 2022
Department of Pharmaceutical Analysis and Nuclear Pharmacy, Faculty of Pharmacy, Comenius University, Bratislava, Slovak Republic.
The possibility to investigate analytes at ultra-low concentration levels still remains a hot topic in bioanalysis. In this area, various preconcentration techniques are an integral part of analytical procedures. When applying electromigration separation techniques, an isotachophoresis has been advantageously employed many times for this purpose.
View Article and Find Full Text PDFUltrasensitive determination of serotonin in human urine by a two dimensional capillary isotachophoresis-capillary zone electrophoresis hyphenated with tandem mass spectrometry.
J Chromatogr A
July 2021
Department of Pharmaceutical Analysis and Nuclear Pharmacy, Faculty of Pharmacy, Comenius University in Bratislava, Odbojarov 10, SK-832 32 Bratislava, Slovak Republic; Toxicological and Antidoping Center, Faculty of Pharmacy, Comenius University in Bratislava, Odbojarov 10, SK-832 32 Bratislava, Slovak Republic. Electronic address:
A two-dimensional capillary isotachophoresis-capillary zone electrophoresis method hyphenated with tandem mass spectrometry was developed and validated for ultrasensitive quantification of serotonin in real human urine samples. Under optimal conditions, the separation was achieved within 12 min (including on-line sample preparation) with the limit of detection of 34 pg mL (due to a large volume sample injection, here 10 µL, and isotachophoretic preconcentration). This concentration limit represents the lowest value for serotonin in comparison to other previously published separation methods employing mass spectrometry detection and applied to urine matrices.
View Article and Find Full Text PDFA fully integrated isotachophoresis with a programmable microfluidic platform.
Talanta
April 2021
Department of Mechanical Engineering, Texas Tech University, Lubbock, TX, 79409, USA; Department of Mechanical Engineering, University of Utah, Salt Lake City, UT, 84112, USA. Electronic address:
Conventional isotachophoresis (ITP) can be used for pre-concentration of a single analyte, but preconcentration of multiple analytes is time consuming due to handling and washing steps required for the extensive buffer optimization procedure. In this work, we present a programmable microfluidic platform (PMP) to demonstrate fully automated optimization of ITP of multiple analytes. By interfacing a PMP with ITP, buffer selection and repetitive ITP procedures were automated.
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