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Maize Streak Virus: Single and Gemini Capsid Architecture.
Viruses
November 2024
Department of Biochemistry and Molecular Biology, College of Medicine Center for Structural Biology, McKnight Brain Institute, University of Florida, Gainesville, FL 32610-0245, USA.
are ssDNA plant viruses whose control has both economical and agricultural importance. Their capsids assemble into two distinct architectural forms: (i) a T = 1 icosahedral and (ii) a unique twinned quasi-isometric capsid. Described here are the high-resolution structures of both forms of the maize streak virus using cryo-EM.
View Article and Find Full Text PDFTargeted Antibacterial Endolysin to Treat Infected Wounds on 3D Full-Thickness Skin Model: XZ.700 Efficacy.
Pharmaceutics
December 2024
VitroScreen s.r.l., In Vitro Innovation Center, Via Mosè Bianchi 103, 20149 Milan, MI, Italy.
Skin wound healing is a physiological process orchestrated by epithelial and mesenchymal cells able to restore tissue continuity by re-organizing themselves and the ECM. This research study aimed to develop an optimized in vitro experimental model of full-thickness skin, to address molecular and morphological modifications occurring in the re-epithelization and wound healing process. Wound healing starting events were investigated within an experimental window of 8 days at the molecular level by gene expression and immunofluorescence of key epidermal and dermal biomarkers.
View Article and Find Full Text PDFDetecting Early Degradation of Wood Ultrastructure with Nonlinear Optical Imaging and Fluorescence Lifetime Analysis.
Polymers (Basel)
December 2024
National Research Council-National Institute of Optics, Largo E. Fermi, 6, 50125 Florence, Italy.
Understanding the deterioration processes in wooden artefacts is essential for accurately assessing their conservation status and developing effective preservation strategies. Advanced imaging techniques are currently being explored to study the impact of chemical changes on the structural and mechanical properties of wood. Nonlinear optical modalities, including second harmonic generation (SHG) and two-photon excited fluorescence (TPEF), combined with fluorescence lifetime imaging microscopy (FLIM), offer a promising non-destructive diagnostic method for evaluating lignocellulose-based materials.
View Article and Find Full Text PDFCryopreservation Protocols and the Associated Ultrastructural Changes in Dormant Buds of .
Plants (Basel)
December 2024
College of Horticulture, Jilin Agricultural University, Changchun 130118, China.
There is an urgent need for the cryopreservation of dormant buds to conserve the genetic resources of woody plants, particularly fruit trees, as this method is less time-consuming and relatively inexpensive. In the present study, three different cryopreservation protocols were tested on dormant buds from three varieties of Rupr. The explants were collected between November 2017 and March 2018.
View Article and Find Full Text PDFThe Ameliorative Effect of Coumarin on Copper Toxicity in : Insights from Growth, Nutrient Uptake, Oxidative Damage, and Photosynthetic Performance.
Plants (Basel)
December 2024
College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Excessive copper (Cu) has become a common physiological disorder restricting the sustainable production of citrus. Coumarin (COU) is a hydroxycinnamic acid that can protect plants from heavy metal toxicity. No data to date are available on the ameliorative effect of COU on plant Cu toxicity.
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