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ATLAS-seq: a microfluidic single-cell TCR screen for antigen-reactive TCRs.
Nat Commun
January 2025
Raymond G. Perelman Center for Cellular and Molecular Therapeutics, Children's Hospital of Philadelphia, Philadelphia, PA, USA.
Discovering antigen-reactive T cell receptors (TCRs) is central to developing effective engineered T cell immunotherapies. However, the conventional technologies for isolating antigen-reactive TCRs (i.e.
View Article and Find Full Text PDFFeatures of the interaction of 5-[4'-(6″-aminopurin-2″-yl)phenyl]-10,15,20-tri(N-methylpyridin-3'-yl)-porphyrin with nucleic acids.
Int J Biol Macromol
December 2024
G.A. Krestov Institute of Solution Chemistry of the Russian Academy of Sciences, Ivanovo, Russia.
Directed synthesis of novel water-soluble asymmetric porphyrins containing in a molecule three cationic fragments and residues of adenine (PorAD) was performed, using metal-complex catalysis method. The interaction of the synthesized porphyrin with the oligonucleotides poly[d(AT)2] and poly[d(GC)2] and double-stranded deoxyribonucleic acid of the calf thymus (ctDNA) was studied by means of spectral and hydrodynamic methods. It was established that PorAD intercalated not only into GC-enriched regions, but also into AT regions.
View Article and Find Full Text PDFOligoDistiller: A Platform Agnostic Software Tool for MS and MS Data Analysis of Complex Oligonucleotides and Their Impurities.
Anal Chem
December 2024
Janssen Pharmaceutica N.V., Turnhoutseweg 30, 2340 Beerse, Belgium.
Oligonucleotides are currently one of the most rapidly advancing classes of therapeutic modalities. Understanding critical quality attributes, such as the impurity profile, stability, potential metabolites, and sequence conformity, is the key to their ultimate success. To obtain the information presented above, liquid chromatography-mass spectrometry (LC-MS) is often employed.
View Article and Find Full Text PDFAptaFluorescence: An aptamer-based fluorescent imaging protocol for biomolecule visualization.
PLoS One
December 2024
School of Biological Sciences, Nanyang Technological University, Singapore, Singapore.
Immunofluorescence is highly dependent on antibody-antigen interactions for accurate visualization of proteins and other biomolecules within cells. However, obtaining antibodies with high specificity and affinity for their target proteins can be challenging, especially for targets that are complex or naturally present at low levels. Therefore, we developed AptaFluorescence, a protocol that utilizes fluorescently labeled aptamers for in vitro biomolecule visualization.
View Article and Find Full Text PDFMulti-Omics Study Reveals Nc886/vtRNA2-1 as a Positive Regulator of Prostate Cancer Cell Immunity.
J Proteome Res
December 2024
Facultad de Ciencias, Universidad de la República, Sección Genómica Funcional, Montevideo 11400, Uruguay.
Noncoding RNA 886 has emerged as a pivotal regulatory RNA with distinct functions across tissues, acting as a regulator of protein activity by directly binding to protein partners. While it is well recognized as a tumor suppressor in prostate cancer, the underlying molecular mechanisms remain elusive. To discover the principal pathways regulated by nc886 in prostate cancer, we used a transcriptomic and proteomic approach analyzing malignant DU145, LNCaP, PC3, and benign RWPE-1 prostate cell line models transiently transfected with in vitro transcribed nc886 or antisense oligonucleotides.
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