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Iron(II/III) Alters the Relative Roles of the Microbial Byproduct and Humic Acid during Chromium(VI) Reduction and Fixation by Soil-Dissolved Organic Matter.
Environ Sci Technol
January 2025
Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtse River), Ministry of Agriculture and Rural Affairs, College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, China.
Though reduction of hexavalent chromium (Cr(VI)) to Cr(III) by dissolved organic matter (DOM) is critical for the remediation of polluted soils, the effects of DOM chemodiversity and underlying mechanisms are not fully elucidated yet. Here, Cr(VI) reduction and immobilization mediated by microbial byproduct (MBP)- and humic acid (HA)-like components in (hot) water-soluble organic matter (WSOM), (H)WSOM, from four soil samples in tropical and subtropical regions of China were investigated. It demonstrates that Cr(VI) reduction capacity decreases in the order WSOM > HWSOM and MBP-enriched DOM > HA-enriched DOM due to the higher contents of low molecular weight saturated compounds and CHO molecules in the former.
View Article and Find Full Text PDFReconstruction of a microbial TNT deep degradation system and its mechanism for reshaping microecology.
J Hazard Mater
January 2025
State Key Laboratory of NBC Protection for Civilian, Beijing 102205, China. Electronic address:
This study is the first to use synthetic biological omics technology to analyze the molecular mechanism underlying deep degradation of TNT, to construct an artificial transformation system to create engineered Escherichia coli bacteria, and to use Bacillus subtilis as an expression host to explore the mechanism driving the reshaping of the deep degradation platform on microecology. Nitroreductase family protein, 2-oxoacid:acceptor oxidoreductase, NADPH-cytochrome P450 reductase, monooxygenase, ring-cleaving dioxygenase, and RraA family protein significantly participated in the reduction-hydroxylation-ring opening cleavage of TNT, achieving deep transformation of TNT to produce pyruvic acid and other products that entered the cellular metabolic cycle. The key toxic metabolic pathways of TNT, 2,4-diamino-6-nitrotoluene, 2,4,6-triaminotoluene, and 2,4,6-trihydroxytoluene are pantothenate and CoA biosynthesis.
View Article and Find Full Text PDFMicrobial synthesis of m-tyrosine via whole-cell biocatalysis.
Enzyme Microb Technol
January 2025
Biotechnology Program, Department of Engineering Technology, Cullen College of Engineering, University of Houston, Houston, TX 77004, United States. Electronic address:
Meta-tyrosine (m-tyrosine), a nonproteinogenic amino acid, has shown significant potential for applications as an herbicide in agriculture and for various medical uses. However, the natural abundance of m-tyrosine is very low, limiting its widespread use. In this study, we successfully achieved microbial production of m-tyrosine by establishing the in vivo enzyme activity of phenylalanine 3-hydroxylase (PacX from Streptomyces coeruleoribudus) in E.
View Article and Find Full Text PDFBiodegradation of Phenol at High Initial Concentration by 3D Strain: Biochemical and Genetic Aspects.
Microorganisms
January 2025
Laboratory of Microbial Enzymology, G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Scientific Center for Biological Research, Russian Academy of Sciences, Prosp. Nauki 5, 142290 Pushchino, Russia.
Phenolic compounds are an extensive group of natural and anthropogenic organic substances of the aromatic series containing one or more hydroxyl groups. The main sources of phenols entering the environment are waste from metallurgy and coke plants, enterprises of the leather, furniture, and pulp and paper industries, as well as wastewater from the production of phenol-formaldehyde resins, adhesives, plastics, and pesticides. Among this group of compounds, phenol is the most common environmental pollutant.
View Article and Find Full Text PDFEfflux Pumps and Porins Enhance Bacterial Tolerance to Phenolic Compounds by Inhibiting Hydroxyl Radical Generation.
Microorganisms
January 2025
State Key Laboratory of Microbial Technology and Institute of Microbial Technology, Shandong University, Qingdao 266237, China.
Phenolic compounds are industrially versatile chemicals that have been successfully produced in microbial cell factories. Unfortunately, most phenolic compounds are highly toxic to cells in specific cellular environments or above a particular concentration because they form a complex with iron and promote hydroxyl radical production in Fenton reactions, resulting in the ferroptosis of cells. Here, we demonstrated that overexpression of efflux pumps and porins, including porins LamB and OmpN, and efflux pumps EmrAB, MdtABC, and SrpB, can enhance phloroglucinol (PG) tolerance by inhibiting the generation of hydroxyl radicals.
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