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Improving Hydrolytic Activity and Enantioselectivity of Epoxide Hydrolase from by Directed Evolution.
Molecules
October 2024
College of Life Sciences, Sichuan Normal University, Chenglong Avenue, Chengdu 610101, China.
Article Synopsis
- * This study created a mutation library using error-prone PCR and DNA shuffling, screening over 15,000 mutants, and eventually identifying 38 mutants with enhanced enzyme activity or enantioselectivity.
- * After extensive testing, one mutant, F3, showed over 95% increased activity compared to the wild type, suggesting its potential as an industrial biocatalyst, supported by molecular docking results.
Detection of Ophidiomyces and Nannizziopsis spp. in the dermal lesions of free-roaming native snakes in Taiwan.
Med Mycol
October 2024
Institute of Veterinary Clinical Sciences, National Taiwan University, Taipei City 106216, Taiwan.
Chrysosporium-related fungi refers to an assemblage of fungi belonging to the Nannizziopsis, Ophidiomyces, and Paranannizziopsis genera. Chrysosporium-related fungi infection results in various skin lesions, such as necrosis and ulcers, in both captive and free-roaming reptiles. To update the prevalence of ophidiomycosis in Taiwan, which was first detected in 2019, we conducted a large-scale ecological survey of free-roaming native snakes with skin lesions in Taiwan.
View Article and Find Full Text PDFTwo novel members of Onygenales, Keratinophyton kautmanovae and K. keniense spp. nov. from soil.
Sci Rep
July 2024
Department of Applied Genetics and Cell Biology, Institute of Microbial Genetics, University of Natural Resources and Life Sciences, Vienna (BOKU), Campus Tulln, Konrad Lorenz Strasse 24, 3430, Tulln an der Donau, Austria.
Two new Keratinophyton species, K. kautmanovae sp. nov.
View Article and Find Full Text PDFCorrelation investigation between core microbe inoculation and the evolution of flavor characteristics during the storage of sturgeon caviar (Acipenser gueldenstaedtii).
Food Res Int
February 2024
College of Food Science and Engineering, Ocean University of China, Qingdao 266404, China. Electronic address:
The volatile and non-volatile compounds were monitored to investigate the microbial evolution associated with the characteristic flavors for sturgeon caviar during refrigeration. The results revealed that the composition of volatile compounds changed significantly with prolonged refrigeration time, especially hexanal, nonanal, phenylacetaldehyde, 3-methyl butyraldehyde, and 1-octen-3-ol. The nonvolatile metabolites were mainly represented by the increase of bitter amino acids (Thr.
View Article and Find Full Text PDFLessons on fruiting body morphogenesis from genomes and transcriptomes of .
Stud Mycol
July 2023
Synthetic and Systems Biology Unit, Biological Research Center, Szeged, 6726, Hungary.
Article Synopsis
- The study focuses on the complex structures called fruiting bodies produced by mushroom-forming fungi, which grow through a genetically programmed process for their development, differentiation, and reproduction.
- The authors identify 921 conserved gene families related to fruiting body morphogenesis, with many previously uncharacterized, and provide novel functional hypotheses based on gene expression and literature reviews.
- A general model of fruiting body development is proposed, detailing an early proliferative phase and a later growth phase involving cell expansion and sporulation, discussing the potential roles of 1,480 genes across several fungal species.
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