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A simple and effective method to remove pigments from heterologous secretory proteins expressed in Pichia pastoris.
Adv Biotechnol (Singap)
February 2024
CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, University of Chinese Academy of Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai, 200030, China.
Pichia pastoris is a popular yeast host for high-level heterologous expression of proteins on an industrial scale owing to its reliable expression, robust growth, high fermentation density, and easy genetic manipulation and cultivation at a relatively low cost. Of particular interest is its high secretion efficiency for small proteins including insulin, human serum albumin, vaccines, enzymes, and llama-derived heavy-chain only antibodies (nanobodies) for pharmaceutical and research applications. However, a recurring challenge in using P.
View Article and Find Full Text PDFStudy of the behaviour of cations in leaching of NCA lithium-ion batteries by electrodialysis.
Environ Technol
January 2025
Department of Chemical Engineering, Polytechnic School, University of Sao Paulo, São Paulo, Brazil.
End-of-life lithium-ion batteries (LIBs) present an opportunity to generate a circular economy through recycling. One of the techniques that can contribute to the purification of leached batteries is electrodialysis. In this work, we present a study of current variation in relation to monovalent (Li), divalent (Ni and Co) and trivalent (Al) cations from the synthetic solution of an NCA-type lithium-ion battery leachate, using electrodialysis membranes (HDX-100 and HDX-200) at three different current densities (12.
View Article and Find Full Text PDFKinetics of ion-mediated directed self-assembly of one-dimensional chains of metal nanoparticles in solution.
Nanoscale
January 2025
Chemical and Biomolecular Engineering, University of Nebraska - Lincoln, Lincoln, Nebraska, 68588, USA.
The synergistic optical, electronic, and chemical properties of metal nanoparticles present in close proximity have potential applications in energy, medicine, and sustainability. Fundamental studies and application development based on spontaneous self-assembly of one-dimensional (1D) chains of metal nanoparticles without external organization agencies have been pursued for over four decades. The spontaneous formation of 1D chains in a solution of stabilized spherical nanoparticles may be driven by the emergence of local anisotropy due to dipolar interaction, representing a trapped non-equilibrium state.
View Article and Find Full Text PDFNucleation-Controlled Crystallization of Chiral 2D Perovskite Single Crystal Thin Films for High-Sensitivity Circularly Polarized Light Detection.
Adv Mater
January 2025
MOE Key Laboratory of Macromolecular Synthesis and Functionalization, International Research Center for X Polymers, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, China.
2D Dion-Jacobson (DJ) chiral perovskite materials exhibit significant promise for developing high-performance circularly polarized light (CPL) photodetectors. However, the inherently thick nature of DJ-phase 2D perovskite single crystal limits their ability to differentiate CPL photons with the two opposite polarization states. In addition, the growth of DJ-phase perovskite single crystal thin films (SCTFs) has proven challenging due to the strong interlayer electronic coupling.
View Article and Find Full Text PDFγ-secretase facilitates retromer-mediated retrograde transport.
J Cell Sci
January 2025
Department of Genetics, Yale School of Medicine, USA.
Retromer mediates retrograde transport of protein cargos from endosomes to the trans-Golgi network (TGN). γ-secretase is a protease that cleaves the transmembrane domain of its target proteins. Although retromer can form a stable complex with γ-secretase, the functional consequences of this interaction are not known.
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