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Isolation, characterization and antimicrobial properties of hepatopancreas lectin of the freshwater crab Oziotelphusanaga.
Protein Expr Purif
October 2024
Centre for Marine Science and Technology, Manonmaniam Sundaranar University, Tirunelveli, India.
Lectins are versatile proteins that specifically recognize and interact with sugar moieties expressed on the cell surface. The potential of lectin in drug targeting and delivery has instigated interest to identify natural lectins. Crabs have been identified as a rich source of lectin because the innate immune system is activated on encounter of pathogens and helps in the production of lectin.
View Article and Find Full Text PDFComprehensive Proteomics Analysis of Polyhydroxyalkanoate (PHA) Biology in Pseudomonas putida KT2440: The Outer Membrane Lipoprotein OprL is a Newly Identified Phasin.
Mol Cell Proteomics
May 2024
BiOrbic - Bioeconomy Research Centre, University College Dublin, Belfield, Dublin, Ireland; UCD Conway Institute, University College Dublin, Belfield, Dublin, Ireland; School of Biomolecular and Biomedical Science, University College Dublin, Belfield, Dublin, Ireland. Electronic address:
Pseudomonas putida KT2440 is an important bioplastic-producing industrial microorganism capable of synthesizing the polymeric carbon-rich storage material, polyhydroxyalkanoate (PHA). PHA is sequestered in discrete PHA granules, or carbonosomes, and accumulates under conditions of stress, for example, low levels of available nitrogen. The pha locus responsible for PHA metabolism encodes both anabolic and catabolic enzymes, a transcription factor, and carbonosome-localized proteins termed phasins.
View Article and Find Full Text PDFDevelopment of a Magnetically-Assisted SERS Biosensor for Rapid Bacterial Detection.
Int J Nanomedicine
January 2024
Medical Technology School of Xuzhou Medical University, Xuzhou, People's Republic of China.
Introduction: Ultrasensitive bacterial detection methods are crucial to ensuring accurate diagnosis and effective clinical monitoring, given the significant threat bacterial infections pose to human health. The aim of this study is to develop a biosensor with capabilities for broad-spectrum bacterial detection, rapid processing, and cost-effectiveness.
Methods: A magnetically-assisted SERS biosensor was designed, employing wheat germ agglutinin (WGA) for broad-spectrum recognition and antibodies for specific capture.
The Pseudomonas aeruginosa lectin LecB modulates intracellular reactive oxygen species production in human neutrophils.
Eur J Immunol
February 2024
Department of Life Sciences, Chalmers University of Technology, Gothenburg, Sweden.
Pseudomonas aeruginosa is a Gram-negative bacterium and an opportunistic pathogen ubiquitously present throughout nature. LecB, a fucose-, and mannose-binding lectin, is a prominent virulence factor of P. aeruginosa, which can be expressed on the bacterial surface but also be secreted.
View Article and Find Full Text PDFPhysicochemical properties and antimicrobial activities of MytiLec-1, a member from the mytilectin family of mussels.
Int J Biol Macromol
December 2023
Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi 6205, Bangladesh. Electronic address:
MytiLec-1, the recombinant form of a mussel lectin from Mytillus galloprovincialis, was purified by affinity chromatography and showed the maximum hemagglutination activity at a temperature range of 10 °C to 40 °C and at pH 7.0 to 9.0.
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