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An ELISA-based assay for determining haemagglutinin potency in egg, cell, or recombinant protein derived influenza vaccines.
Front Immunol
April 2023
Vaccine Product Development, CSL Seqirus Ltd, Parkville, VIC, Australia.
Background: The current compendial assay for haemagglutinin antigen potency in influenza vaccine is the single radial immunodiffusion (SRID) which is time consuming and can lead to delays in release of vaccine. We previously described an alternate capture and detection enzyme linked immunoassay (ELISA) that utilizes sub-type specific, sub-clade cross-reactive monoclonal antibodies (mAbs) that are haemagglutination inhibiting (HAI) and correlate with SRID. The aim of this study is to determine the applicability of ELISA across current platforms for quantitation of seasonal quadrivalent vaccine.
View Article and Find Full Text PDFDevelopment of single-chain Fv against the nucleoprotein of type A influenza virus and its use in ELISA.
J Virol Methods
November 2014
High Security Animal Disease Laboratory, Indian Veterinary Research Institute, Anand Nagar, Bhopal, Madhya Pradesh 462021, India.
Single chain fragment variable (ScFv) antibodies specific to the nucleoprotein (NP) of avian influenza virus (AIV) were developed using a phage display system. The variable heavy (VH) and the variable light (VL) chain gene fragments were derived from spleen cells of Balb/c mouse immunized with a recombinant NP (rNP) antigen (∼63 kDa) of H5N1 influenza virus. The VH and the VL DNA fragments were assembled through a flexible linker DNA to generate ScFv DNA that was cloned subsequently in a phagemid to express ScFv protein in Escherichia coli cells.
View Article and Find Full Text PDFLow pathogenic influenza A virus activity at avian interfaces in Ohio zoos, 2006-2009.
Avian Dis
September 2013
The Ohio State University, Department of Veterinary Preventive Medicine, 1920 Coffey Road, Columbus, OH 43210, USA.
Article Synopsis
- The study investigated influenza A virus activity among birds in four Ohio zoos to understand how the virus from wild aquatic birds might affect captive birds.
- Three low pathogenic avian influenza (AIV) isolates were recovered from zoo birds and environmental samples, indicating that the virus is present in both settings.
- Serological tests showed a prevalence of antibodies in both zoo birds and wild mallards, suggesting a diverse infection and highlighting the need for preventive measures in zoos to protect bird populations.
Antibodies to type A influenza virus in wild waterbirds from Argentina.
J Wildl Dis
July 2010
Southeastern Cooperative Wildlife Disease Study, Department of Population Health, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA.
Limited information exists on avian influenza (AI) virus infection in South American wild birds. As part of a national surveillance program in Argentina, indigenous waterbirds were screened for antibodies to AI virus. From November 2006 to July 2007, serum samples from 540 waterbirds of 12 species were tested for type-specific antibodies to AI virus with the use of a commercially available blocking enzyme-linked immunosorbent assay (bELISA) and the agar-gel immunodiffusion (AGID) test.
View Article and Find Full Text PDFImmunochemical property of human haptoglobin phenotypes: determination of plasma haptoglobin using type-matched standards.
Clin Biochem
September 2007
College of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan, ROC.
Objectives: Haptoglobin (Hp) phenotypes 1-1, 2-1, and 2-2 are associated with inflammatory diseases. Since their biochemical structures are rather heterogeneous, it is necessary to accurately determine the plasma Hp levels.
Design And Methods: Immunodiffusion, immunoturbidimetric, and noncompetitive ELISA were conducted to determine the differences in immunoreactivity among Hp phenotypes and to verify that such difference may significantly affect the outcome of Hp determinations.
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