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http://dx.doi.org/10.1021/jm00300a019 | DOI Listing |
The chromatographic separation of several pituitary proteins on a Mono-Q anion-exchange column is described. The effect of eluent pH and buffer composition on the resolution is demonstrated with several standard proteins. The experimental data indicate that good protein recoveries and resolution can be obtained on this essentially monodisperse microparticulate ion-exchange resin when the pH of the eluent is chosen ca.
View Article and Find Full Text PDFBisbrusatolyl malonate, which was shown previously to be active against P-388 lymphocytic leukemia cell growth, was investigated for inhibitory effects on nucleic acid and protein synthesis. DNA and RNA synthesis as well as protein synthesis were markedly inhibited at 10,25, and 50 mu mole final concentrations in vitro. The major sites of inhibition of nucleic acid synthesis appeared to be DNA polymerase, messenger and transfer RNA polymerases, orotidine-5'-monophosphate decarboxylase, phosphoribosyl pyrophosphate amino transferase, and dihydrofolate reductase.
View Article and Find Full Text PDFPancreatic islets contain calmodulin. This protein activates adenylate cyclase in a subcellular fraction of rat islets in a calcium-dependent fashion. The Km for calmodulin was close to 0.
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