Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Characterization of the GH72 family of Laminarin/Lichenin transferases and their roles in cell wall biogenesis.
Cell Surf
June 2025
Department of Biological Sciences, SUNY University at Buffalo, Buffalo, NY 14260, United States.
In vegetative hyphae, chitin, β-1,3-glucan (laminarin), and a mixed β-1,3-/β-1,4-glucan (lichenin) are the major cell wall polysaccharides. GH72 enzymes have been shown to function as β-1,3-glucanases and glucanosyltransferases and can function in crosslinking β-1,3-glucans together. To characterize the enzymatic activities of the enzymes, we expressed GEL-1 with a HIS6 tag in A chimeric maltose binding protein:GEL-2 was produced in .
View Article and Find Full Text PDFHigh-performance thin-layer chromatography-umu combined with nontarget analysis-a sensitive genotoxicity screening tool applicable for potable water analysis.
Environ Toxicol Chem
January 2025
Zweckverband Landeswasserversorgung, Laboratory for Operation Control and Research, Langenau, Germany.
Monitoring of genotoxic chemicals released into the water cycle or formed through transformation processes is critical to prevent harm to human health. The development of the high-performance thin-layer chromatography (HPTLC)-umu bioassay combines sample separation and detection of genotoxic substances in the low ng/L concentration range. In this study, raw, process, and drinking water samples from 11 different waterworks in Germany were analyzed using the HPTLC-umu.
View Article and Find Full Text PDFComparison of ZnS(Ag) Scintillator and Proportional Counter Tube for Alpha Detection in Thin-Layer Chromatography.
Pharmaceuticals (Basel)
December 2024
Department of Nuclear Medicine, University Hospital Carl Gustav Carus, Technical University Dresden, Fetscherstr. 74, 01307 Dresden, Germany.
(1) : Targeted alpha therapy is an emerging field in nuclear medicine driven by two advantages: overcoming resistance in cancer-suffering patients to beta therapies and the practical application of lower activities of Pb- and Ac-labelled peptides to achieve the same doses compared to beta therapy due to the highly cytotoxic nature of alpha particles. However, quality control of the Pb/Ac-radiopharmaceuticals remains a challenge due to the low activity levels used for therapy (100 kBq/kg) and the formation of several free daughter nuclides immediately after the formulation of patient doses; (2) : The routine alpha detection on thin-layer chromatograms (TLC) of Pb- and Ac-labelled peptides using a MiniScanPRO+ scanner combined with an alpha detector head was compared with detection using an AR-2000 scanner equipped with an open proportional counter tube. Measurement time, resolution and validity were compared for both scanners; (3) : For Ac, the quality control values of the radiochemical purity (RCP) were within the acceptance criteria 2 h after TLC development, regardless of when the TLC probe was taken.
View Article and Find Full Text PDFDevelopment of a Combined 2D-MGD TLC/HPTLC Method for the Separation of Terpinen-4-ol and α-Terpineol from Tea Tree, , Essential Oil.
Biomolecules
January 2025
United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Subtropical Horticulture Research Station (SHRS), Miami, FL 33158, USA.
Tea tree oil (TTO), acquired from (Maiden & Betche) Cheel, Myrtaceae, is a widely utilized essential oil (EO) due to its bioactive properties. The identification and quantification of TTO ingredients is generally performed by GC-MS, which provides the most accurate results. However, in some instances, the cost and time of analysis may pose a challenge.
View Article and Find Full Text PDFPhytochemical and Bioactivity Evaluation of Bee Pollen and Androecia of , , and Species.
Antioxidants (Basel)
December 2024
Department of Pharmacognosy, Faculty of Pharmacy, Yeditepe University, Kayisdagi Cad., Atasehir, 34755 Istanbul, Türkiye.
Qualitative and quantitative differences in the chemical composition between bee pollen originated from (Türkiye and Slovenia), spp. (Türkiye and Slovenia), and spp. (Türkiye) and androecia of , , and (apetalous trees) were evaluated for the first time by new high-performance thin-layer chromatography (HPTLC) and ultra-performance liquid chromatography (UPLC) methods using marker compounds.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!