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Comparison of clinical artificial oocyte activation protocols on mouse egg activation and embryo development.

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D Cohen, Fundación IBYME. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)., Instituto de Biología y Medicina Experimental, Buenos Aires, Argentina.

Artificial oocyte activation (AOA) with Ca2+ ionophores is an experimental procedure that benefits patients who fail to obtain fertilized eggs. However, the impact of non-physiological Ca2+ increases on cellular events involved in egg-embryo transition and early development remains poorly understood. Using the mouse model, this study compares common Ca2+ ionophore protocols applied in clinical practice - one or two exposures to A23187 or a single exposure to ionomycin - focusing on embryonic development and cellular events associated with egg activation.

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  • A major gene locus, SC9.1, was identified as the key regulator of brown seed color in broomcorn millet, a cereal crop known for its diverse seed colors.
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