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Evaluation of micro-colorimetric lipid determination method with samples prepared using sonication and accelerated solvent extraction methods.
Talanta
February 2014
Mid-Continent Ecology Division, National Health and Environmental Effects Research Laboratory, Office of Research and Development, U.S. Environmental Protection Agency, 6201 Congdon Boulevard, Duluth, MN 55804, USA. Electronic address:
Two common laboratory extraction techniques were evaluated for routine use with the micro-colorimetric lipid determination method developed by Van Handel (1985) [2] and recently validated for small samples by Inouye and Lotufo (2006) [1]. With the accelerated solvent extraction method using chloroform:methanol solvent and the colorimetric lipid determination method, 28 of 30 samples had significant proportional bias (α=1%, determined using standard additions) and 1 of 30 samples had significant constant bias (α=1%, determined using Youden Blank measurements). With sonic extraction, 0 of 6 samples had significant proportional bias (α=1%) and 1 of 6 samples had significant constant bias (α=1%).
View Article and Find Full Text PDFStabilization of dentin matrix after cross-linking treatments, in vitro.
Dent Mater
February 2014
Department of Oral Biology, College of Dental Medicine, Georgia Regents University, Augusta, GA, USA. Electronic address:
Objectives: To evaluate the effect of EDC on elastic modulus (E), MMPs activity, hydroxyproline (HYP) release and thermal denaturation temperature of demineralized dentin collagen.
Methods: Dentin beams were obtained from human molars and completely demineralized in 10 wt% H3PO4 for 18 h. The initial E and MMP activity were determined with three-point bending and microcolorimetric assay, respectively.
High-resolution, two-dimensional measurement of dissolved reactive phosphorus in sediments using the diffusive gradients in thin films technique in combination with a routine procedure.
Environ Sci Technol
November 2011
State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, China.
Dissolved reactive phosphorus (DRP) is the most available P form in sediments and often directly controls phytoplankton blooms in aquatic systems. In this study, a novel procedure was developed for two-dimensional (2D) measurement of DRP in sediments at a spatial resolution of 0.45 mm using the diffusive gradients in thin films (DGT) technique with a revised high-capacity binding phase (Zr oxide gel).
View Article and Find Full Text PDFA suite of microplate reader-based colorimetric methods to quantify ammonium, nitrate, orthophosphate and silicate concentrations for aquatic nutrient monitoring.
J Environ Monit
February 2011
Marine Laboratory, Nicholas School of the Environment, Duke University, 135 Duke Marine Lab Rd, Beaufort, North Carolina 28516, USA.
A sensitive, accurate and rapid analysis of major nutrients in aquatic systems is essential for monitoring and maintaining healthy aquatic environments. In particular, monitoring ammonium (NH(4)(+)) concentrations is necessary for maintenance of many fish stocks, while accurate monitoring and regulation of ammonium, orthophosphate (PO(4)(3-)), silicate (Si(OH)(4)) and nitrate (NO(3)(-)) concentrations are required for regulating algae production. Monitoring of wastewater streams is also required for many aquaculture, municipal and industrial wastewater facilities to comply with local, state or federal water quality effluent regulations.
View Article and Find Full Text PDFIn vivo 3T spectroscopic quantification of liver fat content in nonalcoholic fatty liver disease: Correlation with biochemical method and morphometry.
J Hepatol
October 2010
MRI Unit, Medica Sur Hospital & Clinic Foundation, Mexico City, Mexico.
Background & Aims: The clinical application of liver fat quantification has increased in recent years, paralleling the epidemic increase in nonalcoholic fatty liver disease. The aim of this study was to perform a diagnostic evaluation of spectroscopy by comparing its measurement of total lipid content with that from liver biopsies and morphometry in normal subjects and patients with nonalcoholic fatty liver disease.
Methods: Patients with symptomatic cholelithiasis underwent 3T MR cholangiography with spectroscopic quantification of TLC.
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