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Importance: Gastrointestinal nematodiasis poses significant economic losses in the livestock industry due to mortality, morbidity, and decreased production.

Objective: This study examined the prevalence of gastrointestinal nematodiasis in dairy cattle in Central Inner Mongolia, Northern China, the associated risk factors, and the effects on the pepsinogen and gastrin levels.

Methods: Fecal samples (n = 590) were collected from four regions and analyzed using the standard floatation and sedimentation techniques.

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Biomarkers are specific molecular, histological, or physiological characteristics of normal or pathogenic biological processes and are promising in the diagnosis of gastrointestinal nematodes (GINs). Although some biomarkers have been validated for infection by Ostertagia sp. in cattle raised in temperate regions, there is a lack of information for tropical regions.

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Review and Evaluation of Antibody ELISA for Application on Serum Samples in First Season Grazing Calves.

Animals (Basel)

July 2023

Laboratory of Parasitology, Faculty of Veterinary Medicine, Ghent University, Saliburylaan 133, 9820 Merelbeke, Belgium.

The -Ab ELISA assay is widely used as a diagnostic tool for monitoring gastrointestinal (GI) nematodes using milk samples from adult dairy cows. This assay is potentially also useful to analyse serum samples from first-season grazing (FSG) calves, providing a more cost-effective and robust diagnostic technique than the current serum pepsinogen assay. However, a comprehensive evaluation of its use in serum samples from FSG calves has not yet been conducted.

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The diagnostics of ruminant parasites remains one of the cornerstones for parasite control best practices. Field veterinarians have several techniques at their disposal (fecal egg count, coproculture, FAMACHA®, plasma pepsinogen, ELISA-Ostertagia, ELISA-Fasciola, Baermann and ELISA-Lungworm) for the identification and/or quantification of gastrointestinal nematodes, lungworms and liver fluke infecting small ruminants and cattle. Each of these diagnostic tools has its own strengths and weaknesses and is more appropriate for a specific production operation and/or age of the animal (young and adults).

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The rearing system of dairy calves with nurse cows has been developing since 2010 in organic farms in western France. This system allows cow-calf contact until a weaning age close to the natural weaning for cattle and is characterized by an early turnout for calves at around one month of age with their nurse cows and a first grazing season with mixed grazing of calves and adults at a ratio of 2-4 calves per nurse cow. The objectives of this study were to assess the gastrointestinal (GIN) and lungworm infections in such reared calves and their variability during the first grazing season.

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