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Bioassay of cadmium and its effect on differential distribution of dehydrogenases in different brain regions in Labeo rohita (HAM).
Physiol Res
November 1999
Department of Zoology, Regional Institute of Education, National Council of Educational Research and Training, Shyamla Hills, Bhopal, India.
The sublethal effect of cadmium on the specific activities of lactic, malic and succinic dehydrogenases in different brain regions in Labeo rohita (HAM) was assessed with reference to acute, chronic and recovery conditions. Cadmium enhanced succinic, malic and lactic dehydrogenases to a marked extent in the cerebrum from 0 to 12 h exposure. However, a subsequent fall of the above enzymes in some regions was recorded from 12 to 24 h.
View Article and Find Full Text PDFHistochemical evidence of the activity and distribution of glycolysis redox enzymes, tissue respiration and terminal oxidation pattern (dehydrogenase of lactic, malic, succinic and isocitric acids, NAD-N- and NADPh-N-ase, cytochrome oxidase) as well as the levels of the major carbohydrates (glycogen, neutral aminopolysaccharides, glucose) were experimentally studied in the cardiomyocytes of myocardial necrotic, perinecrotic and intact areas in the control and in the experimental material under the administration of terrilitin-nicotinic acid mixture. It was stated that the use of aforementioned mixture contributed to the retention of enzymatic activity and optimal levels of energy formation in the cardiomyocytes of the marginal infarction zone and noticeably prevented the destructive involvement of the considered area as well as the impairment of functional activity of oscillating cardiomyocytes. Therefore, the application of the mixture improved the outcome prognosis in acute myocardial infarction.
View Article and Find Full Text PDFExtrusion of metabolites from baker's yeast during glucose-induced acidification.
Folia Microbiol (Praha)
December 1980
Extrusion of metabolites (glycerol, lactic, malic, and succinic acid) during the medium acidification caused by resting baker's yeast supplied with 200 mM glucose was studied under aerobic and anaerobic conditions and in the absence and presence of 14 mM KCl. The maximum levels of glycerol and of the sum of acids (about 13 and 8 mM, respectively) were attained anaerobically; aerobiosis reduced the levels by 40-50% and the presence of K+ ions by another 10-20%. The time courses of glucose consumption and medium acidification were similar aerobically and anaerobically.
View Article and Find Full Text PDFThe correlation between influenza type A2 and B antigens and the activity of lactic, malic and succinic dehydrogenases, as well as of monoamine oxidase was studied by an original technique allowing simultaneous demonstrations of enzymatic activity and viral antigen. Alterations in enzymatic activity were mainly found in the cellular areas where large amounts of influenza antigen were present.
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