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Establishment of a cell line derived from a mouse fetal liver that has the characteristic to promote the hepatic maturation of mouse embryonic stem cells by a coculture method.
Tissue Eng Part A
December 2009
Department of Surgery, Graduate School of Medicine Kyoto University, Kyoto, Japan.
Stromal cells residing in murine fetal livers have the ability to promote the hepatic maturation of murine embryonic stem cells (ESCs) and hepatic progenitor cells (HPCs) 3848 in vitro. These stromal cells were isolated as the CD49f(+/-)CD45(-)Thy1(+)gp38(+) cell fraction. The present study established a murine fetal liver stromal cell line that induced hepatic maturation in mouse ESCs and HPCs.
View Article and Find Full Text PDFRegulation of Thy-1 gene expression by the methylation of the 5' region of Thy-1 gene and intracellular regulatory factors in immature B cells.
Immunol Invest
June 1992
Osaka University Medical School, Department of Medicine III, Japan.
In c mu- B220+ Thy-1- FL2-52-2, an immature B cell line transformed with a temperature-sensitive mutant of Abelson murine leukemia virus(ts OS-59), Thy-1 antigen expression was induced after the shift of the culture temperature from a permissive(35 degrees C) to a non-permissive temperature(39 degrees C), and a Thy-1+ subclone, FL2-52-2-1 was isolated by limiting dilution. Furthermore, since a population of Thy-1+FL2-52-2-1 lost Thy-1 antigen expression during culture at a non-permissive temperature, Thy-1- FL2-52-2-1-1 was isolated from the cultured cells. Methylation analysis by Southern blotting experiments showed that 5' region of the Thy-1 gene was methylated in Thy-1- FL2-52-2 but demethylated in Thy-1+ FL2-52-2-1 and Thy-1-FL2-52-2-1-1.
View Article and Find Full Text PDFA phenotypically and karyotypically stable human thyroid epithelial line conditionally immortalized by SV40 large T antigen.
Cancer Res
May 1992
Department of Pathology, University of Wales College of Medicine, Heath Park, Cardiff, United Kingdom.
Primary cultures of normal human neonatal thyroid follicular cells were transfected with a plasmid expressing a temperature-sensitive (tsA58) mutant of SV40 large T antigen. An epithelial cell line, designated B-thy-ts.1, was obtained which showed tight temperature-dependent growth.
View Article and Find Full Text PDFTransitory expression of Thy-1 antigen in immature B cell lines.
Immunol Invest
February 1992
Department of Medicine III, Osaka University Medical School, Japan.
In cmu- B220+ Thy-1- murine immature B cells transformed with a temperature-sensitive mutant of Abelson murine leukemia virus, a low level of Thy-1 antigen was transitorily expressed after the shift of the culture temperature from the permissive (35 degrees C) to the non-permissive (39 degrees C) temperature. On the other hand, B220 antigen was persistently expressed regardless of whether the cells were cultured at the permissive or non-permissive temperature. No other T-lineage-specific antigens, CD3, CD4, and CD8 were induced during the culture at the non-permissive temperature.
View Article and Find Full Text PDFAn immune cell population that responds to beta-endorphin and is responsible for protecting nude mice from the fatal consequences of a virus infection of the central nervous system.
J Neuroimmunol
November 1991
Division of Biology, Kansas State University, Manhattan 66506.
Reconstitution of 3- to 4-week-old BALB/c nude (nu/nu) mice with 10(7) syngeneic splenocytes, 48 h before intracerebral inoculation with a temperature-sensitive (ts) mutant of VSV (tsG31 KS5), provided protection from the fatal consequences of clinical disease in 80-90% of the infected animals. Reconstitution of animals with 10(7) splenocytes, first depleted of natural killer (NK) cells with anti-asialo GM1 and complement, also afforded protection against the infectious disease. Depletion of T-lymphocytes with anti-thy-1.
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