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Thriving in adversity: Understanding how maize seeds respond to the challenge of combined cold and high humidity stress.
Plant Physiol Biochem
December 2024
College of Agronomy, Jilin Agricultural University, 2888 Xincheng St, Changchun, 130118, Jilin, PR China; Institute of Agricultural Resource and Environment, Jilin Academy of Agricultural Sciences, 1363 Shengtai St, Changchun, 130033, Jilin, PR China. Electronic address:
Extreme conditions, such as cold and high humidity in northeast China's high-latitude maize region, can hinder crop yield and stability during the vegetative stage. However, there is a paucity of research examining the effects of simultaneous cold and high humidity stress on plant responses. In this study, we characterized the acclimation of JD558 (cold- and high humidity-sensitive hybrid) and JD441 (cold- and high humidity-tolerant hybrid) to stress at sowing caused by cold (4 °C), high humidity (25%), and their combined stress for five days, using physiological measurements and metabolomics during the stress treatments and recovery stages.
View Article and Find Full Text PDFPotential of nanopore sequencing for tuberculosis diagnosis and drug resistance detection.
BMC Infect Dis
December 2024
Xi'an Chest Hospital, Xi'an, Shaanxi Province, China.
Objectives: This study evaluates the effectiveness of nanopore sequencing for accurate detection of Mycobacterium tuberculosis pathogens and drug resistance mutations in clinical specimens.
Methods: A retrospective analysis of 2,421 specimens from suspected tuberculosis patients admitted to Xi'an Chest Hospital from 2022 to 2023 was conducted, with 131 specimens undergoing via real-time, fluorescence-based quantitative Polymerase Chain Reaction (qPCR), simultaneous amplification and testing RNA (RNA), Mycobacterium culture, Mycobacterium smear, and nanopore sequencing. Employing clinical tuberculosis diagnoses as the gold standard, sensitivity, specificity, positive predictive value, negative predictive value, concordance rate, and Kappa coefficient were measured for the five detection techniques.
Direct detection of 4-dimensions of SARS-CoV-2: infection (vRNA), infectivity (antigen), binding antibody, and functional neutralizing antibody in saliva.
Sci Rep
December 2024
School of Dentistry, University of California Los Angeles, 10833 Le Conte Ave., 73-022 CHS, Los Angeles, CA, 90095-1668, USA.
We developed a 4-parameter clinical assay using Electric Field Induced Release and Measurement (EFIRM) technology to simultaneously assess SARS-CoV-2 RNA (vRNA), nucleocapsid antigen, host binding (BAb) and neutralizing antibody (NAb) levels from a drop of saliva with performance that equals or surpasses current EUA-approved tests. The vRNA and antigen assays achieved lower limit of detection (LOD) of 100 copies/reaction and 3.5 TCID₅₀/mL, respectively.
View Article and Find Full Text PDFHigh levels of fibrotic tumor components are associated with recurrence and intratumoral immune status in advanced colorectal cancer patients.
Sci Rep
December 2024
Department of General Surgical Science, Graduate School of Medicine, Gunma University, 3-39-22 Showa-machi, Maebashi, Gunma, 371-8511, Japan.
The importance of collagen and elastin remains incompletely understood concerning tumor immunity in cancer tissues. This study explored the clinical significance of collagen and elastin deposition on tumor immunity in advanced colorectal cancer patients. The collagen and elastin contents were assessed simultaneously using elastic van Gieson (EVG) histochemical staining.
View Article and Find Full Text PDFAssessing Wound Healing in Vivo Using a Dual-Function Phosphorescent Probe Sensitive to Tissue Oxygenation and Regenerating Collagen.
ACS Appl Mater Interfaces
December 2024
Institute of Translational Medicine, Faculty of Health Sciences & Ministry of Education Frontiers Science Center for Precision Oncology, University of Macau, Taipa 999078, Macau, China.
Levels of tissue oxygenation and collagen regeneration are critical indicators in the early evaluation of wound healing. Traditionally, these factors have been assessed using separate instruments and different methodologies. Here, we adopt the spatially averaged phosphorescence lifetime approach using Re-diimine complexes (Re-probe) to enable simultaneous quantification of these two critical factors in healing wounds.
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