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Heart in Focus: Advancing Pericardial Effusion Diagnosis With Point-of-Care Ultrasound.
Cureus
December 2024
Emergency, Hospital de Braga, Braga, PRT.
Pericardial effusion refers to the accumulation of fluid within the pericardial sac, the double-layered membrane surrounding the heart. It can be caused by various medical conditions and may lead to serious complications if not diagnosed and managed promptly. Point-of-care ultrasound (POCUS) has emerged as a valuable tool in the clinical evaluation of pericardial effusions, offering real-time visualization and aiding in the assessment of its size, characteristics, and potential hemodynamic impact.
View Article and Find Full Text PDFMapping photoisomerization dynamics on a three-state model potential energy surface in bacteriorhodopsin using femtosecond stimulated Raman spectroscopy.
Chem Sci
January 2025
School of Physical Science and Technology, ShanghaiTech University Shanghai 201210 China
The process of proton translocation in , triggered by light, is powered by the photoisomerization of all--retinal in bacteriorhodopsin (bR). The primary events in bR involving rapid structural changes upon light absorption occur within subpicoseconds to picoseconds. While the three-state model has received extensive support in describing the primary events between the H and K states, precise characterization of each excited state in the three-state model during photoisomerization remains elusive.
View Article and Find Full Text PDFDevelopment and evaluation of optimized PCR and indirect ELISA for the detection of in dairy cows.
Front Vet Sci
January 2025
College of Veterinary Medicine, Shandong Agricultural University, Tai'an, China.
Introduction: () is a Gram-negative opportunistic pathogen, whose increasing virulence and antibiotic resistance negatively impact dairy cow health and productivity, raising concerns in livestock health management. To mitigate this risk, rapid and reliable diagnostic methods for detection are essential. Currently, detection methods for are underdeveloped, prompting us to develop both pathogenic and serological detection methods, including an optimized PCR technique and an indirect enzyme-linked immunosorbent assay (I-ELISA).
View Article and Find Full Text PDFA novel ddPCR™ assay for eDNA detection and quantification of Greater Amberjack and three congeners in US waters: challenges and application to fisheries independent surveys.
PeerJ
January 2025
School of Biological, Environmental, and Earth Sciences, University of Southern Mississippi, Hattiesburg, MS, United States.
Background: Four species support recreational and commercial fisheries along the U.S. Atlantic Ocean and the Gulf of Mexico, with the Gulf of Mexico stock being overfished for over three decades.
View Article and Find Full Text PDFLiquid biopsy for Renal Cell Carcinoma: A comprehensive review of techniques, applications, and future prospects.
Kidney Cancer
February 2024
University of California San Diego, San Diego, CA, USA.
Liquid biopsy techniques have developed rapidly in recent years and demonstrated success in cancer detection, disease characterization, and ongoing disease monitoring. These components, including circulating tumor cells (CTCs), circulating tumor DNA (ctDNA), and cell-free DNA (cfDNA), offer minimally invasive diagnostic tools that provide valuable insights into the genomic landscape of tumors. Its applications have expanded to include various malignancies, including renal cell carcinoma (RCC).
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