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Telomemore enables single-cell analysis of cell cycle and chromatin condensation.
Nucleic Acids Res
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Laboratory for Molecular Infection Medicine Sweden (MIMS), Umeå University, Biomedicinbyggnaden 6K och 6L, Umeå universitetssjukhus, 901 87, Umeå, Sweden.
Single-cell RNA-seq methods can be used to delineate cell types and states at unprecedented resolution but do little to explain why certain genes are expressed. Single-cell ATAC-seq and multiome (ATAC + RNA) have emerged to give a complementary view of the cell state. It is however unclear what additional information can be extracted from ATAC-seq data besides transcription factor binding sites.
View Article and Find Full Text PDFIsolation and characterization of a β-galactosidase from for industrial processing.
JDS Commun
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Department of Food Science and Technology, The Ohio State University, Columbus, OH 43210.
In this study, a thermostable β-galactosidase from OSU-PECh-4A has been isolated through diafiltration and size-exclusion chromatography. The enzyme consists of a heterodimer with a molecular mass of 110 kDa, with a small and large subunit of 36 and 74 kDa, respectively. The Michaelis constant (K) and maximum velocity (V) values for lactose and -nitrophenyl-β-d-galactopyranoside (NPG) hydrolysis were, respectively, 29.
View Article and Find Full Text PDFIn this study, we demonstrated an inspection system utilizing reflection phase microscopy to enhance both depth range and field of view (FOV). By implementing a dual-wavelength method, we achieved a maximum expected depth range of 7.7 µm.
View Article and Find Full Text PDFDeep eutectic solvents in analysis, delivery and chemistry of pharmaceuticals.
Int J Pharm
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Department of Analytical Chemistry, Applied Chemometrics and Molecular Modelling, Vrije Universiteit Brussels (VUB), Laarbeeklaan 103, Brussels 1090 Belgium. Electronic address:
Deep eutectic solvents (DES) have an emerging scientific role, assisting modern pharmaceutics. They are uniquely supporting the resolution of crucial issues, such as the effective extraction and isolation of bio-actives. They act as media and catalysts for pharmaceutical drug synthesis, and as green solvents and modifiers in pharmaceutical analysis.
View Article and Find Full Text PDFWhich factors influence the transition towards a healthy and sustainable food environment in Dutch hospitals? A qualitative view from stakeholders.
BMC Med
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Consumption & Healthy Lifestyles Group, Wageningen University & Research, Wageningen, The Netherlands.
Background: Hospitals fulfill an important exemplary role in promoting health and well-being. It is therefore crucial to have a supportive food environment that stimulates healthy and sustainable food choices of patients, staff, and visitors. This qualitative study aimed to identify factors influencing the implementation of long-lasting actions to enhance the healthiness and sustainability of the food environment in the hospital setting in the Netherlands, from the perspective of different stakeholders.
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