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Kinetically controlled irreversible unfolding of esterase from Clostridium acetobutylicum: Thermal deactivation kinetics and structural studies.
Int J Biol Macromol
January 2025
Applied and Industrial Microbiology Laboratory, Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences, Indian Institute of Technology, Madras, Chennai 600036, India. Electronic address:
This study involves the thermal characterization of Ca-Est, an esterase from Clostridium acetobutylicum which has been previously found to exhibit maximum specific activity at 60 °C. In the present study, Ca-Est showed maximum stability at 30 °C with almost 75 % of its initial activity being retained after incubation for 5 h and the stability decreased with increasing temperature. Analysis of the thermodynamic parameters revealed that the deactivation of Ca-Est is endothermic and enthalpically favored.
View Article and Find Full Text PDFRiveting Nucleation Enabled Long Cycling Life Calcium Metal Anodes.
Adv Mater
January 2025
Hefei National Research Center for Physical Sciences at the Microscale, Department of Materials Science and Engineering, University of Science and Technology of China, Hefei, 230026, P. R. China.
Calcium metal batteries with high capacity and low cost are promising alternatives to Li-ion batteries for large-scale energy storage. However, its development is crucially impeded by the irreversible Ca metal anode, which is highly associated with uncontrollable Ca plating/stripping. Here, we report a new riveting strategy to regulate the nucleation and growth of a Ca metal anode in the 3D structure of a carbon nanotube film (CNF) by introducing in situ-formed Na metal mediators.
View Article and Find Full Text PDFIdentification and Transcriptome Analysis of with Excellent Low Temperature Resistance.
Int J Mol Sci
December 2024
Co-Innovation Centre for Sustainable Forestry in Southern China, Forestry and Grassland, College of Soil and Water Conservation, Nanjing Forestry University, Nanjing 210037, China.
is one of the most destructive quarantine pests, causing irreversible damage to pine trees. However, the unexpected identification of pine wilt disease in Northern China indicates that can survive under low temperatures. In this study, we analyzed the reproductivity variations among 18 different isolates, and SC13 was identified to have excellent low temperature resistance.
View Article and Find Full Text PDFFundamental Limits of an Irreversible Heat Engine.
Entropy (Basel)
December 2024
Center for Advanced Control and Smart Operations, Nanjing University, Suzhou 215163, China.
We investigated the optimal performance of an irreversible Stirling-like heat engine described by both overdamped and underdamped models within the framework of stochastic thermodynamics. By establishing a link between energy dissipation and Wasserstein distance, we derived the upper bound of maximal power that can be delivered over a complete engine cycle for both models. Additionally, we analytically developed an optimal control strategy to achieve this upper bound of maximal power and determined the efficiency at maximal power in the overdamped scenario.
View Article and Find Full Text PDFRevisions of the Phenomenological and Statistical Statements of the Second Law of Thermodynamics.
Entropy (Basel)
December 2024
Istituto Nazionale di Alta Matematica (INdAM), 00185 Rome, Italy.
The status of the Second Law of Thermodynamics, even in the 21st century, is not as certain as when Arthur Eddington wrote about it a hundred years ago. It is not only about the truth of this law, but rather about its strict and exhaustive formulation. In the previous article, it was shown that two of the three most famous thermodynamic formulations of the Second Law of Thermodynamics are non-exhaustive.
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