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Effects of CO and liquid digestate concentrations on the growth performance and biomass composition of and microalgal strains.
Front Bioeng Biotechnol
January 2025
APESA Pôle valorisation, Montardon, France.
This study evaluated the growth performance of and microalgae cultivated in diluted liquid digestate supplemented with CO, comparing their efficiency to that of a conventional synthetic media. The presence of an initial concentration of ammonium of 125 mg N-NH .L combined with the continuous injection of 1% v/v CO enhanced the optimal growth responses and bioremediation potential for both strains in 200-mL cultures.
View Article and Find Full Text PDFMicrobial lysates as low-cost serum replacements in cellular agriculture media formulation.
Food Res Int
February 2025
Department of Chemical & Biological Engineering, Tufts University Medford MA USA; Tufts University Center for Cellular Agriculture (TUCCA), Tufts University Medford MA USA. Electronic address:
Cultivated meat, the process of generating meat in vitro without sacrificing animals, is a promising alternative to the traditional practice of livestock agriculture. However, the success of this field depends on finding sustainable and economical replacements for animal-derived and expensive fetal bovine serum (FBS) that is typically used in cell culture processes. Here, we outline an effective screening process to vet the suitability of microbial lysates to support the growth of immortalized bovine satellite cells (iBSCs) and mackerel (Mack1) cells.
View Article and Find Full Text PDFIn vitro sperm generation from immature mouse testicular tissue using plasma rich in growth factors.
Stem Cell Res Ther
January 2025
Department of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Background: Culture medium enriched with Knockout serum replacement (KSR) can produce in vitro mouse sperm, but it is inefficient, strain-specific and contains bovine products, which limits its use in the human clinic. The study aimed to optimize the culture medium for testicular tissue by using plasma rich in growth factors (PRGF) as a serum supplement, addressing the limitations of KSR.
Methods: Immature testicular tissues from NMRI mice were cultured for 14 days to identify the optimal PRGF concentration using histological analysis and tubular integrity scoring.
Effect of rapamycin-eluting stents on in-stent restenosis and early inflammatory response in coronary artery narrowing animal models.
J Cardiothorac Surg
January 2025
The First Department of Cardiology, Beidahuang Industry Group General Hospital, Harbin, 150000, Heilongjiang Province, China.
Objective: it was to evaluate the efficacy and safety of rapamycin-eluting stents at different doses in the treatment of coronary artery narrowing in miniature pigs.
Methods: a total of 20 miniature pigs were randomly assigned into four groups: S1 group (low-dose rapamycin-coated stent, 55 µg/mm), S2 group (medium-dose rapamycin-coated stent, 120 µg/mm), S3 group (high-dose rapamycin-coated stent, 415 µg/mm), and D0 group (bare metal stent). The stent size was 3.
Optimization of chemical transfection in airway epithelial cell lines.
BMC Biotechnol
January 2025
Centre for Heart Lung Innovation, St. Paul's Hospital, Providence Healthcare Research Institute, University of British Columbia, 1081 Burrard St, Vancouver, BC, V6Z 1Y6, Canada.
Background: Chemical transfection is a widely employed technique in airway epithelium research, enabling the study of gene expression changes and effects. Additionally, it has been explored for its potential application in delivering gene therapies. Here, we characterize the transfection efficiency of EX-EGFP-Lv105, an EGFP-expressing plasmid into three cell lines commonly used to model the airway epithelium (1HAEo-, 16HBE14o-, and NCI-H292).
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