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The emergence of East Asian spring ephemerals and the unique ecosystem can be attributed primarily to vicariance, brought about by the Quaternary rifting of the Okinawa Trough, the formation of the East China Sea, and the isolation of the island chains of Ryukyu, Japan, and Taiwan from the Asian continent. The northern forests of Japan, dominated by and the associated , present a captivating display of spring-flowering ephemerals, including , , , and . Among these, is also considered part of the spring ephemerals.

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Introduction: The establishment of a high-throughput quantification approach for waterborne pathogenic protozoa and helminths is crucial for rapid screening and health risk assessment.

Methods: We developed a high-throughput quantitative polymerase chain reaction (HT-qPCR) assay targeting 19 waterborne protozoa and 3 waterborne helminths and validated its sensitivity, specificity, and repeatability. The assay was then applied to test various environmental media samples.

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Butterfly classification and species discovery using genomics.

Taxon Rep Int Lepid Surv

October 2023

Department of Biophysics, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX 75390-9050, USA.

Genomic sequencing of worldwide butterfly fauna followed by phylogenetic analysis of protein-coding genes informs butterfly classification throughout the taxonomic hierarchy, from families to species. As a rule, we attribute the same taxonomic rank to more prominent clades of comparable divergence (i.e.

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During macrofungal surveys in 2019-2024, several specimens belonging to the family Psathyrellaceae were collected from the bed of the Indus River, Punjab, Pakistan. Phylogenetic analyses, based on ITS, LSU, and tef-1α sequences and morpho-anatomical study, confirmed the novelty and placement of three taxa in the genus . They are described as , , and .

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Genomic analysis reveals new species and subspecies of butterflies.

Taxon Rep Int Lepid Surv

December 2023

Department of Biophysics, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX 75390-9050, USA.

Large-scale genomic sequencing of butterfly taxa reveals new findings that are presented here. While we focus on detecting species by comparative genomics and define subspecies as groups of populations genetically differentiated from each other but not as strongly as species (i.e.

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