Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/0003-9861(73)90546-8 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Molecular cloning, characterization and in silico analysis of a thermostable β-glucosidase enzyme from Putranjiva roxburghii with a significant activity for cellobiose.
Phytochemistry
August 2017
Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, 247 667, India. Electronic address:
The native Putranjiva roxburghii family 1 glycoside hydrolase enzyme showed β-D-fucosidase activity in addition to β-D-glucosidase and β-D-galactosidase activities reported in our previous study. A single step concanvalin A affinity chromatography for native PRGH1 improved the yield and reduced the purification time. The PRGH1 gene was cloned and overexpressed in E.
View Article and Find Full Text PDFIdentification and characterization of a novel secreted glycosidase with multiple glycosidase activities in Streptococcus intermedius.
J Bacteriol
August 2014
Department of Biological Science and Technology, Institute of Technology and Science, The University of Tokushima Graduate School, Tokushima, Japan
Streptococcus intermedius is a known human pathogen and belongs to the anginosus group (S. anginosus, S. intermedius, and S.
View Article and Find Full Text PDFbeta-D-Galactosidase from Paenibacillus thiaminolyticus catalyzing transfucosylation reactions.
Glycobiology
January 2010
Department of Biochemistry and Microbiology, ICT Prague, Prague, 166 28, Czech Republic.
A genomic library of bacterial strain Paenibacillus thiaminolyticus was constructed and the plasmid DNA of the clone, containing the gene encoding beta-d-galactosidase with beta-d-fucosidase activity, detected by 5-bromo-4-chloro-3-indoxyl beta-d-galactopyranoside, was sequenced. Cells of Escherichia coli BL21 (DE3) were used for production of the enzyme in the form of a histidine-tagged protein. This recombinant fusion protein was purified using Ni-NTA agarose affinity chromatography and characterized by using p-nitrophenyl beta-d-fucopyranoside (K(m) value of (1.
View Article and Find Full Text PDFA procedure has been developed for simultaneous determination of the activity of a-L-fucosidase (EC 3.2.1.
View Article and Find Full Text PDFA method has been developed for simultaneous determination of the activity of alpha-L-fucosidase (EC 3.2.1.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!