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Mechanistic Investigation into the Phase Separation Behavior of Soluplus in the Presence of Biorelevant Media.
Mol Pharm
March 2025
School of Pharmacy, University College Cork, College Road, Cork County, T12 R229 Cork , Ireland.
More than a decade since its introduction, the polymeric excipient Soluplus continues to receive considerable attention for its application in the development of amorphous solid dispersions (ASDs) and its utility as a solubilizer for drugs exhibiting solubility limited absorption. While it is well-recognized that Soluplus forms micelles, the impact of its lower critical solution temperature of approximately 40 °C remains an underexplored aspect. This study investigated the phase behavior of Soluplus in fasted-state simulated intestinal fluid (FaSSIF-V1).
View Article and Find Full Text PDFMycorrhizal fungus BJ1, a new species of sp.: its biological characteristics and promoting effect on seed germination of .
Front Plant Sci
February 2025
College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China.
Mycorrhizal fungi have been shown to promote seed germination and seedling growth in Orchidaceae plants. In the present study, a mycorrhizal fungus designated as BJ1 was isolated from the roots of (Thunb.) Reiehb.
View Article and Find Full Text PDFStandardization of lipid sample preparation for monitoring phospholipase activity.
Arch Biochem Biophys
March 2025
Departamento de Química, Instituto de Ciências Exatas, Universidade Federal de Minas Gerais, 31270-901, Belo Horizonte, Brazil. Electronic address:
Phospholipase enzymes, such as A1, A2, B, and D, are found in the venom of venomous animals, including brown spiders. Phospholipase D (PLD) isoforms from brown spider venom can cause dermonecrosis, hemolysis, and nephrotoxicity. New methods to monitor PLD activity are essential for understanding its mechanisms and molecular characteristics.
View Article and Find Full Text PDFBiophysical insights into sodium lauroyl sarcosine induced amyloid fibrillation of human serum albumin.
Spectrochim Acta A Mol Biomol Spectrosc
February 2025
Department of Biotechnology, Jamia Millia Islamia, New Delhi 110025, India. Electronic address:
Sodium lauroyl sarcosinate (SLS), an anionic surfactant is known to solubilize recombinant proteins during purification processes. Though SLS has been shown to induce amyloid fibrillation in proteins, the specific role of SLS in amyloid formation remains less understood. Despite its well-established use in protein solubilization, further research is needed to clarify its potential influence on amyloid fibrillation pathways.
View Article and Find Full Text PDFScreening of plant growth-promoting rhizobacteria and their growth-promoting activities for Stipa purpurea in alpine grassland.
J Appl Microbiol
March 2025
Qinghai Academy of Agriculture and Forestry Sciences, Qinghai University, No. 251 Ningda Road, Chengbei District, Xining City, Qinghai Province, Xining 810016, China.
Aims: Stipa purpurea is one of the dominant grass species in alpine grassland and plays a crucial role in safeguarding the ecosystem and restoring degraded grassland. To enhance the population dominance of S. purpurea and effectively prevent further grassland degradation.
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