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MiRNA-223-5p inhibits hypoxia-induced apoptosis of BMSCs and promotes repair in Legg-Calvé-Perthes disease by targeting CHAC2 and activating the Wnt/β-catenin signaling pathway.
PLoS One
January 2025
School of Clinical Medicine, Guizhou Medical University, Guiyang, China.
Legg-Calvé-Perthes disease (LCPD) involves femoral head osteonecrosis caused by disrupted blood supply, leading to joint deformity and early osteoarthritis. This study investigates the role of miRNA-223-5p in regulating hypoxia-induced apoptosis and enhancing osteogenesis in bone marrow mesenchymal stem cells (BMSCs). Utilizing a juvenile New Zealand white rabbit model of LCPD established through femoral neck ligation, we transfected BMSCs with miR-223-5p mimics, inhibitors, and controls, followed by hypoxic exposure.
View Article and Find Full Text PDFAn Enigmatic Wild Passerine Mortality Event in the Eastern United States.
Vet Sci
January 2025
Southeastern Cooperative Wildlife Disease Study, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA.
The ability to rapidly respond to wildlife health events is essential. However, such events are often unpredictable, especially with anthropogenic disturbances and climate-related environmental changes driving unforeseen threats. Many events also are short-lived and go undocumented, making it difficult to draw on lessons learned from past investigations.
View Article and Find Full Text PDFMulti-stressor dynamics: Effects of marine heatwave stress and harmful algal blooms on juvenile mussel (Perna canaliculus) survival and physiology.
Sci Total Environ
January 2025
Cawthron Institute, 98 Halifax Street East, Nelson 7010, New Zealand. Electronic address:
In New Zealand, the frequency and intensity of marine heatwaves (MHWs) and blooms of the harmful algal species, Alexandrium pacificum, are increasing in areas where there are natural reefs and commercial farms of the mussel, Perna canaliculus. In this study, we assessed the whole organism, tissue and molecular-level response of juvenile (spat) P. canaliculus exposed to these abiotic and biotic stressors, alone and together.
View Article and Find Full Text PDFAchieving reliable patient reported outcomes collection to measure health care improvement in a learning health network: lessons from pediatric rheumatology care and outcomes improvement network.
Front Pediatr
January 2025
Division of Rheumatology, The Hospital for Sick Children, Toronto, ON, Canada.
Introduction: Data from the Pediatric Rheumatology Care and Outcomes Improvement Network (PR-COIN) registry suggests that reliable collection of patient-reported outcomes (PROs) varies across sites. The objective of this study was to better understand the practices of collecting PROs at PR-COIN sites.
Methods: A REDCap survey was sent to the lead representative for each PR-COIN site.
Newly developed genomic SSR markers revealed the population structure and genetic characteristics of abaca ( Nee).
BioTechnologia (Pozn)
December 2024
Institute of Crop Science, College of Agriculture and Food Science, University of the Philippines Los Baños, Philippines.
Abaca ( Nee) is the primary source of manila hemp fiber, a vital industrial product for the country. Previous studies have relied on molecular markers designed for other species or distant genera like rice, limiting accurate genetic characterization and germplasm conservation. To address this, we developed 50 genome-specific molecular markers based on the recently released whole genome sequence assembly of Abaca var.
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