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Incorporation of radiophosphorus from labeled oligodeoxynucleotides into RNA of mycoplasma in cell cultures.
Antisense Res Dev
December 1992
Clinical Pharmacology Branch, NCI, NIH, Bethesda, Maryland.
We have found that various mycoplasma species quickly and efficiently incorporate radiophosphorus into their RNA from labeled oligonucleotides added to the medium. The label can be in any of several positions in an oligodeoxynucleotide, and incorporation also occurs efficiently from labeled RNA. Mycoplasmas also incorporate the radiolabel when they infect a mammalian cell culture; the host cells do not.
View Article and Find Full Text PDFModulation of 32Pi incorporation into phospholipids of polymorphonuclear leukocytes by ionophore A23187.
Biochim Biophys Acta
November 1978
The effects of ionophore A23187 on the incorporation of 32Pi into phospholipids and on 45Ca2+ uptake and release by polymorphonuclear leukocytes were examined. A23187 increased 32Pi incorporation into phosphatidic acid, phosphatidylglycerol, phosphatidylserine, and the phosphoinositides. It also promoted a rapid burst uptake and release of 45Ca2+ by leukocytes.
View Article and Find Full Text PDFThe incorporation of radioprecursors into intestinal epithelial cell proteins and phospholipids was studied to assess the role of protein and phospholipid synthesis and/or turnover in the cessation of the absorption of macromolecules (closure). Radiophosphorus incorporation into cellular phospholipids was enhanced when pinocytosis was stimulated. The specific activity of cellular and brush border phospholipids and specific phosphatides increased during the period of active endocytosis.
View Article and Find Full Text PDFOvarian 32P incorporation in hypophysectomized Heteropneustes fossilis in response to pituitary gland extract pooled from same species and mammalian gonadotropic preparations were studied. Maximum 32P uptake by ovary was obtained when a tracer dose of radiophosphorus was given 30 minutes after LH injection and fish were sacrificed 12 hours after the tracer shot. A log-dose response was observed between ovarian 32P uptake and gonadotropic content of pituitary extract or LH in hypophysectomized H.
View Article and Find Full Text PDFRadiophosphorus incorporation by ovary was the criterion used for the assessment of ovarian activity in H. fossilis. Prolactin administration at different dose levels failed to elicit any obvious change in ovarian 32P uptake.
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