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Plasmid Library Construction From Genomic DNA.
Curr Protoc
January 2025
New England Biolabs, Ipswich, Massachusetts.
Functional genomic approaches have been effective at uncovering the function of uncharacterized genes and identifying new functions for known genes. Often these approaches rely on an in vivo screen or selection to associate genes with a phenotype of interest. These selections and screens are dependent upon the expression of proteins encoded in genomic DNA from an expression vector, such as a plasmid.
View Article and Find Full Text PDFPreparation, structural property, and antioxidant activities of a novel pectin polysaccharide from the flowers of Linn.
Front Nutr
January 2025
Prefabricated Dish Industry Development Research Institute, Zhejiang Dong Fang Polytechnic, Wenzhou, China.
Introduction: Oxidative stress, triggered by an imbalance between reactive oxygen species (ROS) production and cellular antioxidant defense mechanisms, is implicated in various pathological conditions. Plant-derived polysaccharides have gained significant attention as potential natural antioxidants due to their biocompatibility, biodegradability, and structural versatility.
Methods: This study focuses on the purification, structural characterization, and antioxidant activities of a novel pectin polysaccharide (HFPS) isolated from the flowers of Linn.
Evaluation of host immune responses to Mycobacteriophage Fionnbharth by route of delivery.
Virol J
January 2025
Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, WA, USA.
For much of the last decade, tuberculosis (TB) was the leading cause of mortality due to an infectious pathogen (Mycobacterium tuberculosis, M.tb). Approximately 1.
View Article and Find Full Text PDFSL70, a Novel Exopolysaccharide Producer from Traditional Sourdough Fermentation of Einkorn ( L. ssp. ).
Food Technol Biotechnol
December 2024
Department of Food Engineering, Gumushane University, Baglarbasi Road, 29100 Gumushane, Turkey.
Research Background: Given the potential of microbial exopolysaccharides from lactic acid bacteria in various industrial processes, alternative sources for the isolation of lactic acid bacteria are highly topical. In this study, we used a traditional sourdough from einkorn ( L. ssp.
View Article and Find Full Text PDFPurification, crystal structural characterization of porcine kidney dipeptidyl peptidase IV (PkDPP-IV) and its interaction with oyster derived inhibitory peptide ILAPPER.
Int J Biol Macromol
January 2025
College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, China. Electronic address:
Dipeptidyl peptidase IV (DPP-IV) is an important target enzyme for the treatment of type 2 diabetes mellitus (T2DM). Increasing researchers try to screen DPP-IV inhibitory peptides while the cost of DPP-IV is high. In this study, PkDPP-IV was efficiently purified by acid precipitation, ammonium sulfate salting out and gel filtration chromatography with a purification of 283.
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