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Isolation, purification and structural elucidation of Mellein from endophytic fungus Lasiodiplodia theobromae strain (SJF-1) and its broad-spectrum antimicrobial and pharmacological properties.
Lett Appl Microbiol
December 2022
Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India.
In an on-going investigation of bioactive metabolites producing potential endophytic fungi, the strain Lasiodiplodia theobromae (SJF-1) was isolated from a medicinal plant Syzygium cumini. The cultural, morphological and molecular identification was done with the SJF-1 strain. The obtained gene sequence was deposited in NCBI with accession number MG 938644.
View Article and Find Full Text PDFIdentification of Mellein as a Pathogenic Substance of by UPLC-MS/MS Analysis and Phytotoxic Bioassay.
J Agric Food Chem
August 2021
College of Plant Health and Medicine, Key Laboratory of Integrated Crop Pest Management of Shandong Province, Qingdao Agricultural University, Qingdao 266109, P. R. China.
is a pathogenic fungus that can cause apple ring rot, a destructive apple disease in China. There have been reports on its molecular pathogenesis, but the pathogenic substances still remain unknown. In the present study, instrument analysis including UPLC-high-resolution mass spectrometry (HRMS) and nuclear magnetic resonance showed that fermentation broth contained ()-(-)-mellein, a well-known fungal enantiomer of mellein.
View Article and Find Full Text PDFPhytotoxic Metabolites Isolated from the Causal Agent of the Scabby Canker of Cactus Pear ( L.).
Toxins (Basel)
February 2020
Dipartimento Scienze Chimiche, Università di Napoli Federico II, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Napoli, Italy.
Six phytotoxins were obtained from the culture filtrates of the ascomycete , the causal agent of the scabby canker of cactus pear ( L.) in minor Sicily islands. The phytotoxins were identified as (-)-()-mellein (); (±)-botryoisocoumarin A (); (-)-(3,4)- and (-)-(3,4)-4-hydroxymellein ( and ); (-)-terpestacin (); and (+)-3,4-dihydro-4,5,8-trihydroxy-3-methylisocoumarin, which we named (+)-neoisocoumarin ().
View Article and Find Full Text PDFHigh-performance liquid chromatography-tandem mass spectrometry method for simultaneous detection of ochratoxin A and relative metabolites in Aspergillus species and dried vine fruits.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess
August 2016
a College of Food Science and Nutritional Engineering , China Agricultural University, Beijing , China.
A simple, sensitive and reliable quantification and identification method was developed for simultaneous analysis of ochratoxin A (OTA) and its related metabolites ochratoxin alpha (OTα), ochratoxin B (OTB) and mellein. The method was assessed by spiking analytes into blank culture media and dried vine fruits. Performance was tested in terms of accuracy, selectivity and repeatability.
View Article and Find Full Text PDFAn in planta-expressed polyketide synthase produces (R)-mellein in the wheat pathogen Parastagonospora nodorum.
Appl Environ Microbiol
January 2015
Plant Sciences Division, Research School of Biology, The Australian National University, Canberra, Australian Capital Territory, Australia.
Parastagonospora nodorum is a pathogen of wheat that affects yields globally. Previous transcriptional analysis identified a partially reducing polyketide synthase (PR-PKS) gene, SNOG_00477 (SN477), in P. nodorum that is highly upregulated during infection of wheat leaves.
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