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Extraction of Omega-3 Fatty Acids from Atlantic Sea Cucumber () Viscera Using Supercritical Carbon Dioxide.
Mar Drugs
August 2024
Biorefining and Remediation Laboratory, Department of Process Engineering and Applied Science, Dalhousie University, Halifax, NS B3J 1B6, Canada.
Article Synopsis
- * The optimal extraction parameters identified were 75 °C, 45 MPa pressure, with specific time and solvent ratios, resulting in higher FAs yield (16.30 g/100 g) compared to the conventional Bligh and Dyer method (9.02 g/100 g).
- * Pre-treatment methods like freeze-drying greatly improved FA recovery, achieving nearly 100% yield, while ethanol soaking also enhanced yields, suggesting scCO extraction from sea cucumber viscera is a promising, eco-friendly alternative to traditional techniques
Determination of key volatile fishy substances of sea cucumber powder during the processing and their removal by supercritical fluid extraction.
Food Res Int
August 2024
SKL of Marine Food Processing & Safety Control, National Engineering Research Center of Seafood, Liaoning Province Collaborative Innovation Center for Marine Food Deep Processing, Dalian Technology Innovation Center for Chinese Prepared Food, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China; Academy of Food Interdisciplinary Science, Dalian Polytechnic University, Dalian 116034, Liaoning, China. Electronic address:
More than 40 volatile compounds were detected in sea cucumber powder during the processing (through freeze-dried, desalination, supercritical fluid extraction and ultra-micro grinding) by multiple methods including e-nose, GC-IMS and GC-MS. It has been determined that aldehydes are the predominant volatile substances in the original freeze-dried sample, accounting for about 30 % of the total volatile substances. In addition, we established a supercritical fluid extraction strategy that could efficiently remove the aldehydes from the sea cucumber powder.
View Article and Find Full Text PDFUltrastructural examination of cryodamage in Paracentrotus lividus eggs during cryopreservation.
Sci Rep
April 2024
Centro de Investigación Mariña (CIM), Departamento de Ecoloxía e Bioloxía Animal, Grupo ECOCOST, Universidade de Vigo, 36208, Pontevedra, Spain.
This study examinates the challenges of cryopreserving sea urchin (Paracentrotus lividus) eggs, a task hindered by factors like low membrane permeability and high sensitivity to cryoprotective agents (CPAs). While successful cryopreservation has been achieved for some marine invertebrates, eggs remain problematic due to their unique characteristics. The study explores the impact of various CPAs and cryopreservation techniques on sea urchin eggs, employing scanning and transmission electron microscopy to analyze cellular damage.
View Article and Find Full Text PDFCryopreservation of the collector urchin embryo, Tripneustes gratilla.
Cryobiology
June 2024
Hawai'i Institute of Marine Biology, University of Hawai'i, Kaneohe, HI, 96744, USA; Center for Species Survival, Smithsonian National Zoo and Conservation Biology Institute, Smithsonian Institution, Front Royal, VA, 22630, USA.
The collector urchin, Tripneustes gratilla, is an ecologically important member of the grazing community of Hawai'i's coral reefs. Beyond its ability to maintain balance between native seaweeds and corals, T. gratilla has also been used as a food source and a biocontrol agent against alien invasive algae species.
View Article and Find Full Text PDFCold storage and cryopreservation methods for spermatozoa of the sea urchins Lytechinus pictus and Strongylocentrotus purpuratus.
Dev Dyn
August 2024
Center for Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California San Diego, La Jolla, California, USA.
Background: Sea urchins have contributed greatly to knowledge of fertilization, embryogenesis, and cell biology. However, until now, they have not been genetic model organisms because of their long generation times and lack of tools for husbandry and gene manipulation. We recently established the sea urchin Lytechinus pictus, as a multigenerational model Echinoderm, because of its relatively short generation time of 4-6 months and ease of laboratory culture.
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