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Computational Analysis of the Selective Formation of the C4α-C8' Bond in the Intermolecular Coupling of Catechin Derivatives.
J Org Chem
April 2020
Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939-0398, Japan.
Procyanidin B3 is a natural flavonoid composed of two catechins connected via a C4α-C8' bond. The couplings of catechin derivatives, promoted by Lewis acids, have been widely applied to the syntheses of procyanidin B3 and related flavonoids because the reactions construct the C4α-C8' bond in a highly stereo- and regioselective manner. However, the structural complexity of the catechin derivatives has complicated the exploration of a detailed mechanism for this selectivity.
View Article and Find Full Text PDFOrthogonal separation protocol for the simultaneous preparation of four medically active compounds from Anemarrhenae Rhizoma by sequential polyamide and macroporous resin adsorbent chromatography.
J Sep Sci
August 2016
State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, P. R. China.
The green and efficient preparation of natural products from biomass is considered an important area of interest in pharmaceutical applications. In this study, we aimed to provide a practical example with a popular traditional Chinese medicine, Anemarrhenae Rhizome, and showcase the orthogonal use of column chromatography with polyamide and macroporous adsorption resins to selectively concentrate and efficiently purify four bioactive compounds: neomangiferin (NMF), mangiferin (MF), timosaponin B-II (TS B-II), and timosaponin A-III (TS A-III). First, polyamide T60-100 was employed to fractionalize the crude extracts of Anemarrhenae Rhizome.
View Article and Find Full Text PDFComparison of isolated hepatocytes and tissue slices for study of liver hypothermic preservation/reperfusion injury.
Cryobiology
August 1996
Department of Surgery, University of Wisconsin, Madison, USA.
Simple models are needed that effectively test the variables that may be important in liver preservation. Two such models are isolated hepatocytes and tissue slices. In this study the effects of hypothermic preservation on the viability of hepatocytes (HC) and tissue slices (TS) from rat livers were measured by LDH leakage after cold storage and rewarming.
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