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The establishment of conservation areas is an important strategy for endangered species conservation. In this study, we investigated the distributions of suitable habitat areas for three level 1 endangered Cupressaceae plants (, , and ) in China and used the Marxan model to delineate the priority conservation areas for each species. The results showed that had the broadest suitable growing area under the current climate in China and is followed by , with an area of 91 × 10 km, and had the smallest suitable habitat areas at only 7 × 10 km.

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Nitrogen fertilizer application is an important method for the production of high-quality maize. However, nitrogen fertilizer addition patterns vary according to regional climate, field management practices, and soil conditions. In this study, a meta-analysis was used to quantify the yield effects of nitrogen addition on maize, and meta-regression analysis and a random forest model were used to study the main factors affecting the yield effects of nitrogen addition on maize.

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Specific and sensitive detection of bovine coronavirus using CRISPR-Cas13a combined with RT-RAA technology.

Front Vet Sci

January 2025

Key Laboratory of Animal Medicine at Southwest Minzu University of Sichuan Province, College of Animal Science and Veterinary Medicine, Southwest Minzu University, Chengdu, China.

Introduction: Bovine coronavirus (BCoV) is an important pathogen of enteric and respiratory disease in cattle, resulting in huge economic losses to the beef and dairy industries worldwide. A specific and sensitive detection assay for BCoV is critical to the early-stage disease prevention and control.

Methods: We established a specific, sensitive, and stable assay for BCoV nucleic acid detection based on CRISPR/Cas13a combined with reverse transcription recombinase-aided amplification (RT-RAA) technology.

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Co-methylation networks associated with cognition and structural brain development during adolescence.

Front Genet

January 2025

Tri-Institutional Center for Translational Research in Neuroimaging and Data Science (TReNDS): (Georgia State University, Georgia Institute of Technology, and Emory University), Atlanta, GA, United States.

Introduction: Typical adolescent neurodevelopment is marked by decreases in grey matter (GM) volume, increases in myelination, measured by fractional anisotropy (FA), and improvement in cognitive performance.

Methods: To understand how epigenetic changes, methylation (DNAm) in particular, may be involved during this phase of development, we studied cognitive assessments, DNAm from saliva, and neuroimaging data from a longitudinal cohort of normally developing adolescents, aged nine to fourteen. We extracted networks of methylation with patterns of correlated change using a weighted gene correlation network analysis (WCGNA).

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Background Nasogastric tubes (NGTs) have long been considered standard practice for the management of adhesional small bowel obstructions (ASBOs). However, the evidence to support the routine use of NGTs in ASBO is sparse. This study aims to review outcomes associated with NGT use in a large tertiary centre.

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