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Statistical optimization of pectinases from thermophilic Aspergillus fumigatus BT-4 employing response surface methodology through submerged fermentation using agricultural wastes.
BMC Biotechnol
January 2025
School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, China.
Background: In this study, thermophilic pectinase-producing strains were isolated. Among all the isolates, strain No. 4 was identified as Aspergillus fumigatus BT-4 based on its morphology and 18 S rDNA analysis.
View Article and Find Full Text PDFImmobilization of purified pectinase from Aspergillus nidulans on chitosan and alginate beads for biotechnological applications.
Microb Cell Fact
January 2025
Botany and Microbiology Department, Faculty of Science, Benha University, Benha, Egypt.
Background: Because the process is cost-effective, microbial pectinase is used in juice clearing. The isolation, immobilization, and characterization of pectinase from Aspergillus nidulans (Eidam) G. Winter (AUMC No.
View Article and Find Full Text PDFIsolation and characterization of ɸEcM-vB1 bacteriophage targeting multidrug-resistant Escherichia coli.
BMC Res Notes
January 2025
Department of Microbiology and Immunology, Faculty of Pharmacy, Damanhour University, Damanhour, Egypt.
Objectives: The aim of this study is to screen for, isolate and characterize a bacteriophage designated ɸEcM-vB1 with confirmed lytic activity against multidrug-resistant (MDR) E. coli. Methods done in this research are bacteriophage isolation, purification, titer determination, bacteriophage morphology, host range determination, bacteriophage latent period and burst size determination, genomic analysis by restriction enzymes, and bacteriophage total protein content determination.
View Article and Find Full Text PDFBio-inspired chitosan/polydopamine-nanoparticle based sorbent bead: A versatile platform for separation and HPLC analysis of tetracycline antibiotics from various sample matrix.
J Food Drug Anal
December 2024
Department of Medical Laboratory Science and Biotechnology, Kaohsiung Medical University, Kaohsiung, Taiwan.
This study introduces an innovative bio-based sorbent bead crafted by integrating chitosan (CS) biopolymers, Fe(NO3)3 and polydopamine nanoparticles (PDA NPs) via glutaraldehyde crosslinking. The primary focus of this study was the concurrent separation of diverse tetracycline antibiotics (TCs), followed by rigorous reversed-phase liquid chromatography analysis. The fabricated CS/Fe@PDA sorbent beads were comprehensively characterized using scanning electron microscopy and energy-dispersive X-ray spectroscopy, revealing a surface rich in active carbon (C), nitrogen (N), and oxygen (O) moieties.
View Article and Find Full Text PDFMental health in Germany before, during and after the COVID-19 pandemic.
PLoS One
January 2025
Kyiv School of Economics, Kyiv, Ukraine.
Article Synopsis
- The study analyzes mental health trends in Germany using data from over 18,000 individuals from 2018 to 2022, observing a significant decline during the COVID-19 pandemic.
- The most severe drops in mental health occurred during the Delta variant wave in 2021, with scores remaining below pre-pandemic levels into mid-2022.
- Factors like age, gender, income, and other demographics showed no notable differences in mental health decline, suggesting the pandemic's impact was consistent across various groups.
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