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Sample Preparation of Caenorhabditis elegans for GC-MS-Based Metabolomics in Toxicity Assessment.
Methods Mol Biol
January 2025
Fytagoras BV, Leiden, The Netherlands.
The nematode Caenorhabditis elegans, widely recognized as a model organism due to its ease of breeding and well-characterized genomes, boasts complete digestive, reproductive, and endocrine systems, as well as conserved signaling pathways shared with mammals. It has become an invaluable resource for metabolomics research, particularly in examining responses to chemical or environmental factors and toxicity assessments. In this article, we provide detailed, step-by-step protocols for cultivating C.
View Article and Find Full Text PDFIdentification and Profiling of Primary Metabolites Through GC-MS and Associated Data Processing.
Methods Mol Biol
January 2025
College of Pharmacy, Chung-Ang University, Seoul, Republic of Korea.
This chapter presents a comprehensive approach to profiling plant-derived primary metabolites using metabolomics, highlighting its critical role in decoding the biosynthesis of bioactive plant compounds. It details the utilization of gas chromatography-mass spectrometry (GC-MS) for the effective analysis and profiling of these metabolites. The process, encompassing extraction methods, chemical derivatization, and data processing, is thoroughly outlined.
View Article and Find Full Text PDFExtraction of Essential Oils and Terpene Volatiles from Plants and Identification by GC-MS-Based Techniques.
Methods Mol Biol
January 2025
Department of Cell and Metabolic Biology, Leibniz Institute of Plant Biochemistry, Halle, Germany.
The extraction of plant essential oils (EOs) and analysis by gas chromatography coupled to mass spectrometry (GC-MS) are standard methods when studying aromatic plants and the chemical composition of EOs. Here, two simple methods for the extraction of EO compounds from leaves of Thymus vulgaris are described. Organic solvent extraction and solid-phase microextraction (SPME), respectively, are used and the results of the GC-MS analyses are compared.
View Article and Find Full Text PDFAnalysis key aroma compounds based on the aroma quality and infusion durability of jasmine tea.
Food Chem
January 2025
College of Food Science, National Engineering Research Center of Sugarcane, Fujian Agriculture and Forestry University, Fuzhou 350002, China. Electronic address:
The quality of jasmine tea is closely related to the aroma of the tea infusion and the brew durability of the tea leaves. In this study, the aroma components of jasmine tea infusions subjected to multiple brewings were investigated using sensory evaluation, headspace solid-phase microextraction gas chromatography-mass spectrometry (HS-SPME-GC-MS), and headspace gas chromatography-ion mobility spectrometry (HS-GC-IMS). 179 and 73 volatile compounds were identified by HS-SPME-GC-MS and HS-GC-IMS.
View Article and Find Full Text PDFChemical composition, antibacterial and antioxidant activities of Piper betle and Anethum graveolens essential oils against Methicillin-resistant Staphylococcus aureus clinical isolates.
Braz J Microbiol
January 2025
Amity Institute of Biotechnology, Amity University Uttar Pradesh, Sector-125, Noida, Uttar Pradesh, 201313, India.
Methicillin-resistant Staphylococcus aureus (MRSA) is one of the leading causes of infection worldwide due to its antimicrobial resistance. Plant-derived essential oils (EOs) have undergone extensive observational and clinical research to explore their antimicrobial properties. The present study aimed to check mec A positive MRSA isolates using sequencing analysis, determination of chemical composition using gas chromatography-mass spectroscopy (GC-MS), antioxidant, and antimicrobial activity of Anethum graveolens and Piper betle EOs against the infectious agent MRSA.
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